Design and development of amperometric biosensor for the detection of lead and mercury ions in water matrix-a permeability approach

Analytical and Bioanalytical Chemistry
Manju Bhargavi GumpuJohn Bosco Balaguru Rayappan

Abstract

Intake of water contaminated with lead (Pb2+) and mercury (Hg2+) ions leads to various toxic effects and health issues. In this context, an amperometric urease inhibition-based biosensor was developed to detect Pb2+and Hg2+ions in water matrix. The modified Pt/CeO2/urease electrode was fabricated by immobilizing CeO2nanoparticles and urease using a semi-permeable adsorption layer of nafion. With urea as a substrate, urease catalytic activity was examined through cyclic voltammetry. Further, maximum amperometric inhibitive response of the modified Pt/CeO2/urease electrode was observed in the presence of Pb2+and Hg2+ions due to the urease inhibition at specific potentials of -0.03 and 0 V, respectively. The developed sensor exhibited a detection limit of 0.019 ± 0.001 μM with a sensitivity of 89.2 × 10-3 μA μM-1for Pb2+ions. A detection limit of 0.018 ± 0.003 with a sensitivity of 94.1 × 10-3 μA μM-1was achieved in detecting Hg2+ions. The developed biosensor showed a fast response time (<1 s) with a linear range of 0.5-2.2 and 0.02-0.8 μM for Pb2+and Hg2+ions, respectively. The modified electrode offered a good stability for 20 days with a good repeatability and reproducibility. The developed sensor was used to detect Pb2+and Hg2...Continue Reading

References

Dec 18, 2004·Analytical and Bioanalytical Chemistry·Andrew O SimmRichard G Compton
Apr 24, 2007·Analytical Chemistry·Deivasigamani PrabhakaranHideyuki Matsunaga
Jan 17, 2012·Biosensors & Bioelectronics·Yasumoto DateNaoya Ohmura
Jul 14, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Minh-Phuong Ngoc BuiGi Hun Seong
Dec 12, 2012·Chemistry Central Journal·Nana DuShuisheng Chen
Sep 17, 2013·Journal of Colloid and Interface Science·Noel NesakumarJohn Bosco Balaguru Rayappan
Apr 30, 2014·Journal of Colloid and Interface Science·Mudit SinghJohn Bosco Balaguru Rayappan

❮ Previous
Next ❯

Citations

Dec 1, 2018·Analytical and Bioanalytical Chemistry·Mohammad Reza MahmoudianYatimah Alias
Jul 1, 2018·Analytical and Bioanalytical Chemistry·Shengpan XuHe Huang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.