Design and discovery of 2-(4-(1H-tetrazol-5-yl)-1H-pyrazol-1-yl)-4-(4-phenyl)thiazole derivatives as cardiotonic agents via inhibition of PDE3

Bioorganic & Medicinal Chemistry
Li-Min DuanChun-Juan Jia

Abstract

A series of novel 2-(4-(1H-tetrazol-5-yl)-1H-pyrazol-1-yl)-4-(4-phenyl)thiazole derivatives, 6(a-o) were designed, synthesized and evaluated for inhibitory activity against human PDE3A and PDE3B. In PDE3 assay, entire set of targeted analogs showed considerable inhibition of PDE3A (IC50=0.24 ± 0.06-16.42 ± 0.14 μM) over PDE3B (IC50=2.34 ± 0.13-28.02 ± 0.03 μM). Among the synthesized derivatives, compound 6d exhibited most potent inhibition of PDE3A with IC50=0.24 ± 0.06 μM than PDE3B (IC50=2.34 ± 0.13 μM). This compound was further subjected for evaluation of cardiotonic activity (contractile and chronotropic effects) in comparison with Vesnarinone. Results showed that, it selectively modulates the force of contraction (63%± 5) rather than frequency rate (23% ± 2) at 100 μM. Docking study of above compound was also carried out in the active site of PDE3 protein model to give proof to the mechanism of action of designed inhibitor. Further, in sub-acute toxicity experiment in Swiss-albino mice, it was found to be non-toxic up to 100mg/kg dose for 28days.

References

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Dec 15, 2012·Circulation·Alan S GoUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee

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Citations

Feb 9, 2018·Journal of Cardiovascular Development and Disease·Matthew MovsesianEmilio Hirsch
Feb 13, 2018·RSC Advances·Ajay L ChandgudeAlexander Dömling
Aug 8, 2019·Nature Reviews. Drug Discovery·George S BaillieMichy P Kelly
Feb 2, 2019·Current Topics in Medicinal Chemistry·Christophe TratratMarina Soković
Jan 8, 2021·European Journal of Medicinal Chemistry·Nathalia Fonseca NadurArthur Eugen Kümmerle
Nov 23, 2019·European Journal of Medicinal Chemistry·Daniel SzulczykMarta Struga

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