Design and evaluation of microfluidic devices for two-dimensional spatial separations

Journal of Chromatography. a
E DavydovaPeter J Schoenmakers

Abstract

Various designs of chips for comprehensive two-dimensional spatial liquid chromatography were investigated. The performance of these chips was initially evaluated using computational fluid dynamics (CFD). A bifurcating distributor with an angle of 140° between branches was implemented in order to achieve a homogeneous velocity field. The cross-sectional area of the channels of the flow distributor was fixed at 0.5×0.5mm, which allows a robust micromilling technique to be used for chip manufacturing. Experiments were performed with chips featuring purposely introduced imperfections in the structure of the bifurcating flow distributor to study its capacity of overcoming potential local clogging. Split peaks were observed when 75% of one of the flow channels was obstructed, in line with the CFD predictions. The main bottlenecks for the performance of the spatial two-dimensional chips were identified, viz. sample injected in the first dimension diverging into the flow distributor and channel discretization (i.e., remixing of first-dimension separation peaks because of finite number of second-dimension channels). Solutions to the former problem were studied by applying a flow resistance in the vertical segments that formed the outle...Continue Reading

References

Dec 27, 2005·Journal of Chromatography. a·Peter J SchoenmakersWim M C Decrop
Jul 28, 2007·Journal of Chromatography. a·Paola DugoLuigi Mondello
Sep 25, 2007·Journal of Chromatography. a·Dwight R StollSarah C Rutan
Jul 19, 2011·Analytica Chimica Acta·Dominique J D VanhouttePeter J Schoenmakers
Aug 30, 2012·Analytical Chemistry·Elena UliyanchenkoPeter J Schoenmakers
Jul 23, 2013·Journal of Chromatography. a·Andrea Nagy, Attila Gaspar
Apr 23, 2014·Analytical Chemistry·Peter Schoenmakers, Petra Aarnoutse
Aug 5, 2014·Journal of Chromatography. a·Rudy J VonkPeter J Schoenmakers
Oct 20, 2014·Analytical and Bioanalytical Chemistry·Pavel JanderaMagda Staňková
Jan 20, 2015·Journal of Separation Science·Bert WoutersSebastiaan Eeltink

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Citations

Feb 1, 2021·Analytica Chimica Acta·Thomas ThemelisSebastiaan Eeltink
Apr 21, 2021·Chemical Reviews·Noor AbdulhussainPeter Schoenmakers
May 30, 2021·Analytical and Bioanalytical Chemistry·Sam WoutersAndrea F G Gargano
Oct 23, 2019·Analytical Chemistry·Alexander S KaplitzJames P Grinias

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