PMID: 9171890Dec 1, 1996Paper

Design and expression of a stable bispecific scFv dimer with affinity for both glycophorin and N9 neuraminidase

Molecular Immunology
J L AtwellP J Hudson

Abstract

We have designed and produced a stable bispecific scFv dimer (bisFv) by non-covalent association of two hybrid VH-VL pairs derived from an anti-neuraminidase antibody (NC10) and an anti-glycophorin antibody (1C3). The bisFv dimer was demonstrated to have binding activity to the two respective target antigens and was evaluated as a reagent for rapid whole blood agglutination assays. The bisFv was expressed in the periplasm of Escherichia coli, from a secretion vector which comprised two cistrons in tandem under the control of a single lac promoter, inducible with IPTG. Each cistron encoded one of the hybrid VH-VL pairs, with V domains separated by a linker region encoding the five amino acids, Gly4Ser. The short linker region was designed to prevent association of VH and VL regions of the same molecule and favour the formation of dimers. The protein synthesized from each hybrid scFv cistron was directed to the E. coli periplasm by the inclusion of distinctive signal secretion sequences preceding each hybrid gene; from pel B of Erwinia cartovora and from gene III of fd phage. The bisFv was affinity-purified from culture supernatants via the C-terminal tag epitope FLAG and was shown, by FPLC on a Superose 6 column, to be consisten...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1978·Nucleic Acids Research·E BeckM Takanami
Jan 1, 1991·Methods in Enzymology·J S HustonH Oppermann
Apr 8, 1991·Journal of Immunological Methods·K M WilsonB E Kemp
Apr 1, 1991·Journal of Virological Methods·J L McKimm-BreschkinA A Kortt
Feb 13, 1990·Biochemistry·R GlockshuberA Plückthun
Jan 15, 1990·The Medical Journal of Australia·D B RylattD Stapleton
Jun 12, 1989·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P M ColmanR G Webster
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
May 25, 1989·Nucleic Acids Research·D Güssow, T Clackson
Jun 25, 1985·Nucleic Acids Research·P CarterG Winter
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·A ZdanovM Cygler
Feb 24, 1995·Journal of Molecular Biology·D NeriG Winter
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M MackP Kufer
Dec 15, 1994·Structure·O PerisicR L Williams
Feb 10, 1995·Journal of Molecular Biology·P PackA Plückthun
Jan 1, 1994·Advances in Immunology·D R Burton, C F Barbas
Jan 1, 1994·Annual Review of Immunology·G WinterH R Hoogenboom
Feb 1, 1994·Molecular Immunology·D P McGregorW J Harris
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P HolligerG Winter
Feb 1, 1996·Immunotechnology : an International Journal of Immunological Engineering·A M WuJ E Shively

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Citations

Aug 4, 1998·International Journal of Cancer. Journal International Du Cancer·S M KipriyanovM Little
Sep 15, 2000·Journal of Immunological Methods·B E Power, P J Hudson
Feb 27, 2001·Journal of Immunological Methods·A TodorovskaP J Hudson
Jan 29, 2000·Journal of Immunological Methods·P J Hudson, A A Kortt
Nov 26, 1998·Biosensors & Bioelectronics·K Alfthan
Sep 22, 2001·Biomolecular Engineering·A A KorttP J Hudson
Nov 18, 2010·Journal of Microbiological Methods·Fang-Yu HsuHwan-You Chang
Jul 15, 2015·Oncoimmunology·Carter M SuryadevaraJohn H Sampson
Jun 27, 1998·Journal of Molecular Biology·R L MalbyP M Colman
Jan 11, 2017·MAbs·Ulrich Brinkmann, Roland E Kontermann
May 22, 2020·Journal of Drug Targeting·Vala KafilYadollah Omidi
Jan 11, 2016·Applied Biochemistry and Biotechnology·Shima Moradi-KalbolandiMohammad Ali Shokrgozar
Feb 1, 2014·Microbiology Spectrum·Kin-Ming LoBjörn Hock
Nov 12, 2018·ACS Biomaterials Science & Engineering·Srinivas AbbinaJayachandran N Kizhakkedathu

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