Design and implementation of an array of micro-electrochemical detectors for two-dimensional liquid chromatography--proof of principle

Journal of Chromatography. a
Jude A AbiaGeorges Guiochon

Abstract

Simultaneous two-dimensional liquid chromatography (2D-LC) is an implementation of two-dimensional liquid chromatography which has the potential to provide very fast, yet highly efficient separations. It is based on the use of time x space and space x space separation systems. The basic principle of this instrument has been validated long ago by the success of two-dimensional thin layer chromatography. The construction of a pressurized wide and flat column (100 mm x 100 mm x 1 mm) operated under an inlet pressure of up to 50 bar was described previously. However, to become a modern analytical method, simultaneous 2D-LC requires the development of detectors suitable for the monitoring of the composition of the eluent of this pressurized planar, wide column. An array of five equidistant micro-electrochemical sensors was built for this purpose and tested. Each sensor is a three-electrode system, with the working electrode being a 25 microm polished platinum micro-electrode. The auxiliary electrode is a thin platinum wire and the reference electrode an Ag/AgCl (3M sat. KCl) electrode. In this first implementation, proof of principle is demonstrated, but the final instrument will require a much larger array.

References

May 20, 1992·Journal of Chromatography·J G Alvarez, J C Touchstone
Mar 4, 2008·Journal of Chromatography. a·Khaled S Mriziq, Georges Guiochon
Mar 14, 2008·Journal of Chromatography. a·Georges GuiochonR Andrew Shalliker
Apr 25, 2008·Journal of Chromatography. a·Khaled S MriziqGeorges Guiochon
Mar 10, 2009·Journal of Chromatography. a·Jude A AbiaGeorges A Guiochon

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