Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines
Rohollah NasiriSamad Ahadian

Abstract

Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction-expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of t...Continue Reading

References

Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dino Di CarloMehmet Toner
Jul 2, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Steven J CohenNeal J Meropol
Oct 14, 2009·Lab on a Chip·Dino Di Carlo
Apr 27, 2010·Analytical and Bioanalytical Chemistry·Daniel R GossettDino Di Carlo
Dec 24, 2010·Journal of Chromatography. a·Myung Gwon LeeJe-Kyun Park
Aug 21, 2013·Lab on a Chip·Majid Ebrahimi WarkianiJongyoon Han
Jun 7, 2014·Annual Review of Biomedical Engineering·Joseph M Martel, Mehmet Toner
Jun 18, 2015·Journal of Laboratory Automation·Ling X KongMarc Madou
Nov 20, 2015·Lab on a Chip·Jun ZhangWeihua Li
Jan 23, 2016·Lab on a Chip·Chao LiuGuoqing Hu
May 28, 2017·Scientific Reports·Sam H AuMehmet Toner
Oct 3, 2017·Sensors and Actuators. B, Chemical·Zeta Tak For YuJianping Fu
Jan 13, 2018·Advanced Drug Delivery Reviews·Si-Jie HaoSi-Yang Zheng
Mar 2, 2018·Analytical Chemistry·Xinjie ZhangZhonghua Ni
Feb 6, 2016·Micromachines·Minghui TangHo-Pui Ho
Jan 17, 2019·Cancers·Arutha KulasingheChamindie Punyadeera
Mar 12, 2020·Materials Today. Bio·N AshammakhiA Khademhosseini
Jun 18, 2020·Advanced Healthcare Materials·Ahmet ErdemNureddin Ashammakhi
Jul 6, 2020·Biomaterials·Nureddin AshammakhiAli Khademhosseini
Oct 20, 2020·Advanced Materials Technologies·Elham DavoodiNureddin Ashammakhi

❮ Previous
Next ❯

Citations

Jul 2, 2021·Electrophoresis·Arash Dalili, Mina Hoorfar

❮ Previous
Next ❯

Methods Mentioned

BETA
Magnetic-Activated
Fluorescent-Activated Cell Sorting
chip

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.