Design and Synthesis of a Ratiometric Photoacoustic Probe for In Situ Imaging of Zinc Ions in Deep Tissue In Vivo.

Analytical Chemistry
Chaobang ZhangShulin Zhao

Abstract

As a noninvasive deep-tissue imaging technique, photoacoustic (PA) imaging has great application potential in biomedicine and molecular diagnosis. The zinc ion (Zn2+), which is a necessary metal ion in the human body, plays a very important role in the regulation of gene transcription and metalloenzyme function. The imbalance of Zn2+ homeostasis is also associated with a variety of neurological diseases. Therefore, it is critically important to accurately image the steady-state changes of Zn2+ in vivo. However, no PA imaging method is currently available for Zn2+. To this end, we designed and synthesized the first PA probe of Zn2+, namely, CR-1 for in situ ratiometric imaging of Zn2+ in deep tissue in vivo. The CR-1molecule, combined with Zn2+, weakened the conjugation system of the π-electron in the CR-1 molecule, which resulted in the blue shift of its absorption peak from 710 nm to 532 nm. The PA signal intensity decreased at 710 nm and increased at 532 nm, and the ratiometric PA signal at these two wavelengths (PA532/PA710) showed a good linear relationship with the concentration of Zn2+ in the range of 0-50 μM, with a detection limit as low as 170 nM. Furthermore, this probe exhibits extremely fast responsiveness, is highl...Continue Reading

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Citations

Aug 10, 2020·Chembiochem : a European Journal of Chemical Biology·Shichao Wang, Xuanjun Zhang
Apr 28, 2021·Journal of the American Chemical Society·Melissa Y LuceroJefferson Chan
Sep 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Melissa Y LuceroJefferson Chan

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