Design and Synthesis of an Artificial Pulmonary Pleura for High Throughput Studies in Acellular Human Lungs

Cellular and Molecular Bioengineering
Darcy E WagnerDaniel J Weiss

Abstract

Whole organ decellularization of complex organs, such as lungs, presents a unique opportunity for use of acellular scaffolds for ex vivo tissue engineering or for studying cell-extracellular matrix interactions ex vivo. A growing body of literature investigating decellularizing and recellularizing rodent lungs has provided important proof of concept models and rodent lungs are readily available for high throughput studies. In contrast, comparable progress in large animal and human lungs has been impeded owing to more limited availability and difficulties in handling larger tissue. While the use of smaller segments of acellular large animal or human lungs would maximize usage from a single lung, excision of small acellular segments compromises the integrity of the pleural layer, leaving the terminal ends of blood vessels and airways exposed. We have developed a novel pleural coating using non-toxic ionically crosslinked alginate or photocrosslinked methacrylated alginate which can be applied to excised acellular lung segments, permits inflation of small segments, and significantly enhances retention of cells inoculated through cannulated airways or blood vessels. Further, photocrosslinking methacrylated alginate, using eosin Y a...Continue Reading

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Citations

Feb 22, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Patrick N CharronRachael A Oldinski
Apr 22, 2015·Annals of the American Thoracic Society·Daniel J WeissUNKNOWN ATS Subcommittee on Stem Cells and Cell Therapies
Mar 2, 2016·Biotechnology Progress·Ying Lei, Zannatul Ferdous
Jul 6, 2016·ACS Applied Materials & Interfaces·Spencer L FennRachael A Floreani
Aug 11, 2016·Annals of the American Thoracic Society·Darcy E WagnerUNKNOWN ATS Subcommittee on Stem Cells and Cell Therapies
Jun 27, 2017·ACS Applied Materials & Interfaces·Spencer L FennRachael A Floreani
Jun 28, 2018·PloS One·Sean M WrennDaniel J Weiss
Jun 8, 2018·European Respiratory Review : an Official Journal of the European Respiratory Society·Sarah E Gilpin, Darcy E Wagner
Jun 16, 2018·The European Respiratory Journal·Martina M De SantisDarcy E Wagner
Sep 3, 2017·Biotechnology Journal·Katja ZscheppangAndreas C Hocke
Aug 29, 2018·Journal of Materials Science. Materials in Medicine·Jennifer N EtterRachael A Oldinski
Dec 11, 2020·Advanced Materials·Martina M De SantisDarcy E Wagner
Aug 26, 2021·Regenerative Medicine·Seyed Hossein MahfouziGhassem Amoabediny

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