Design and Validation of In-Source Atmospheric Pressure Photoionization Hydrogen/Deuterium Exchange Mass Spectrometry with Continuous Feeding of D2 O

Journal of the American Society for Mass Spectrometry
Thamina ActerSunghwan Kim

Abstract

In this study, continuous in-source hydrogen/deuterium exchange (HDX) atmospheric pressure photoionization (APPI) mass spectrometry (MS) with continuous feeding of D2O was developed and validated. D2O was continuously fed using a capillary line placed on the center of a metal plate positioned between the UV lamp and nebulizer. The proposed system overcomes the limitations of previously reported APPI HDX-MS approaches where deuterated solvents were premixed with sample solutions before ionization. This is particularly important for APPI because solvent composition can greatly influence ionization efficiency as well as the solubility of analytes. The experimental parameters for APPI HDX-MS with continuous feeding of D2O were optimized, and the optimized conditions were applied for the analysis of nitrogen-, oxygen-, and sulfur-containing compounds. The developed method was also applied for the analysis of the polar fraction of a petroleum sample. Thus, the data presented in this study clearly show that the proposed HDX approach can serve as an effective analytical tool for the structural analysis of complex mixtures. Graphical abstract ᅟ.

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Citations

Apr 1, 2018·Mass Spectrometry Reviews·Yury KostyukevichEugene Nikolaev
Oct 26, 2018·Journal of the American Society for Mass Spectrometry·O Tara LiyanageElyssia S Gallagher
Jan 5, 2021·Journal of the American Society for Mass Spectrometry·Julius PavlovAthula B Attygalle
Nov 16, 2018·Analytical Chemistry·Edouard NiyonsabaHilkka I Kenttämaa
Dec 4, 2021·Mass Spectrometry Reviews·Annelaure DamontJean-Claude Tabet

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