Sep 13, 2019

Design, assembly, characterization, and operation of double-stranded interlocked DNA nanostructures

Nature Protocols
Julián ValeroMichael Famulok

Abstract

Mechanically interlocked DNA nanostructures are useful as flexible entities for operating DNA-based nanomachines. Interlocked structures made of double-stranded (ds) DNA components can be constructed by irreversibly threading them through one another to mechanically link them. The interlocked components thus remain bound to one another while still permitting large-amplitude motion about the mechanical bond. The construction of interlocked dsDNA architectures is challenging because it usually involves the synthesis and modification of small dsDNA nanocircles of various sizes, dependent on intrinsically curved DNA. Here we describe the design, generation, purification, and characterization of interlocked dsDNA structures such as catenanes, rotaxanes, and daisy-chain rotaxanes (DCRs). Their construction requires precise control of threading and hybridization of the interlocking components at each step during the assembly process. The protocol details the characterization of these nanostructures with gel electrophoresis and atomic force microscopy (AFM), including acquisition of high-resolution AFM images obtained in intermittent contact mode in liquid. Additional functionality can be conferred on the DNA architectures by incorpora...Continue Reading

  • References
  • Citations1

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations1

Citations

Mentioned in this Paper

Size
Pseudorotaxanes
Molecular Probe Techniques
Nanostructured Materials
Light
Microscopy, Atomic Force
Site
Desires
SWI3 protein, S cerevisiae
Structure

Related Feeds

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.

Related Papers

Accounts of Chemical Research
Stefan-S Jester, Michael Famulok
Chemical Communications : Chem Comm
Damian AckermannMichael Famulok
Journal of the American Chemical Society
Tao Li, Michael Famulok
Topics in Current Chemistry
Fuan WangItamar Willner
Nature Nanotechnology
Damian AckermannMichael Famulok
© 2020 Meta ULC. All rights reserved