Design-based estimation of surface area in thick tissue sections of arbitrary orientation using virtual cycloids

Journal of Microscopy
A M GokhaleP R Mouton

Abstract

Surface area is a first-order stereological parameter with important biological applications, particularly at the intersection of biological phases. To deal with the inherent anisotropy of biological surfaces, state-of-the-art design-based methods require tissue rotation around at least one axis prior to sectioning. This paper describes the use of virtual cycloids for surface area estimation of objects and regions in thick, transparent tissue sections cut at any arbitrary (convenient) orientation. Based on the vertical section approach of Baddeley et al., the present approach specifies the vertical axis as the direction of sectioning (i.e. the direction perpendicular to the tissue section), and applies computer-generated cycloids (virtual cycloids) with their minor axis parallel to the vertical axis. The number of surface-cycloid intersections counted on focal planes scanned through the z-axis is proportional to the surface area of interest in the tissue, with no further assumptions about size, shape or orientation. Optimal efficiency at each x-y location can be achieved by three virtual cycloids orientated with their major axes (which are parallel to the observation planes) mutually at an angle of 120 degrees. The major practi...Continue Reading

Citations

Jun 17, 2011·American Journal of Nephrology·Behzad Najafian, Michael Mauer
Sep 18, 2009·Experimental Dermatology·Søren KampTomas Norman Dam
Mar 7, 2015·Cell and Tissue Research·Lucie Kubínová, Jiří Janáček
Jan 5, 2019·PloS One·Bruno Grosselli LaniaPaulo Eduardo Neves Ferreira Velho
Oct 27, 2017·Journal of Cutaneous Pathology·Fabiane Leonel UtinoMaria Letícia Cintra
Jul 9, 2020·The Journal of Comparative Neurology·Lutz Slomianka
Feb 20, 2021·Journal of Neuroscience Methods·Palak DavePeter R Mouton
Oct 15, 2020·Histochemistry and Cell Biology·Lars KnudsenMatthias Ochs
Feb 5, 2013·Cold Spring Harbor Protocols·Mark J West

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