Design, construction and evaluation of multi-epitope antigens for diagnosis of Lyme disease

Annals of Agricultural and Environmental Medicine : AAEM
Eva SchreterovaLucia Borszekova Pulzova

Abstract

Introduction and objective. Lyme disease (LD) is the most common vector-borne disease in the temperate zone of the Northern Hemisphere. Diagnosis of LD is mainly based on clinical symptoms supported with serology (detection of anti-<i>Borrelia</i> antibodies) and is often misdiagnosed in areas of endemicity. In this study, the chimeric proteins (A/C-2, A/C-4 and A/C-7.1) consisting of B-cell epitopes of outer surface proteins OspA and OspC from <i>Borrelia</i> genospecies prevalent in Eastern Slovakia, were designed, over-expressed in <i>E. coli</i>, and used to detect specific anti-<i>Borrelia</i> antibodies in serologically characterized sera from patients with Lyme-like symptoms to evaluate their diagnostic potential. Results showed that chimeras vary in their immuno-reactivity when tested with human sera. Compared with the results obtained from a two-tier test, the application of recombinant multi-epitope chimeric proteins as diagnosis antigens, produced fair agreement in the case of A/C-2 (0.20<κ<0.40) and good agreement (0.60<κ<0.80) when A/C-7.1 was used as capture antigen. Chimera A/C-4 were excluded from further study due to loss of reactivity with OspA-specific antibodies. The combination of specific B-cell epitopes f...Continue Reading

Citations

Jul 25, 2019·Recent Patents on Biotechnology·Patrícia A F RibeiroAlexsandro S Galdino

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Related Papers

The New England Journal of Medicine
J BerglundR Norrby
The New England Journal of Medicine
S E SchutzerP K Coyle
Journal of Clinical Microbiology
M Gomes-SoleckiR J Dattwyler
© 2022 Meta ULC. All rights reserved