Design, expression, and characterization of a novel dendritic cell-targeted proteins

Biochemical and Biophysical Research Communications
Yu WangJi Yun Yu

Abstract

In vivo approaches to inducing an effective immune response focus on targeted antigen (Ag) delivery to dendritic cells (DCs). In this study, we developed a new method of targeting plasmid DNA and/or the antigen (Ag)-antibody (Ab) complex to DCs via the DC receptor DEC-205, also known as cluster of differentiation CD205. We cloned and expressed a recombinant protein composed of mouse DEC-205-specific single-chain fragment variable region (mDEC-205-scFv), the streptococcal protein G (SPG) IgG-binding domain and cationic peptide (CP), which named mDEC205-scFv-SPG-CP (msSC). In vitro, the recombinant protein msSC can specifically bind to DCs through the section of mDEC-205-scFv, and bound the Ag-Ab complex via SPG as well as plasmid DNA through electrostatic bonding with CP in vitro. In addition, msSC functioned in a manner similar to anti-DEC-205 monoclonal Ab and bound to mouse bone marrow-derived DCs. It was demonstrated in vivo that msSC can target plasmid DNA to DCs, resulting in efficient uptake and expression. Moreover, msSC can form a complex with pGL3-CMV and transport it to draining lymph nodes when injected in vivo. These results indicate that msSC can be used as a carrier protein for vaccine delivery to DCs via formatio...Continue Reading

References

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Citations

Oct 15, 2020·Signal Transduction and Targeted Therapy·Yetian DongFangfang Zhou
Sep 8, 2020·Angewandte Chemie·Christoph O FranckLjiljana Fruk
Oct 13, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Chen CuiLixiong Zhang

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