Abstract
In vivo approaches to inducing an effective immune response focus on targeted antigen (Ag) delivery to dendritic cells (DCs). In this study, we developed a new method of targeting plasmid DNA and/or the antigen (Ag)-antibody (Ab) complex to DCs via the DC receptor DEC-205, also known as cluster of differentiation CD205. We cloned and expressed a recombinant protein composed of mouse DEC-205-specific single-chain fragment variable region (mDEC-205-scFv), the streptococcal protein G (SPG) IgG-binding domain and cationic peptide (CP), which named mDEC205-scFv-SPG-CP (msSC). In vitro, the recombinant protein msSC can specifically bind to DCs through the section of mDEC-205-scFv, and bound the Ag-Ab complex via SPG as well as plasmid DNA through electrostatic bonding with CP in vitro. In addition, msSC functioned in a manner similar to anti-DEC-205 monoclonal Ab and bound to mouse bone marrow-derived DCs. It was demonstrated in vivo that msSC can target plasmid DNA to DCs, resulting in efficient uptake and expression. Moreover, msSC can form a complex with pGL3-CMV and transport it to draining lymph nodes when injected in vivo. These results indicate that msSC can be used as a carrier protein for vaccine delivery to DCs via formatio...Continue Reading
References
May 15, 2003·Vaccine·Joana G BrandãoTanja D de Gruijl
May 4, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Alexander V PereboevJerry L Blackwell
Apr 15, 2005·Molecular Immunology·Caroline DemangelWarwick J Britton
Aug 3, 2005·Cancer Research·Karsten MahnkeAlexander H Enk
Jan 17, 2007·Vaccine·Xin YaoYu-Mei Wen
Jan 24, 2007·Vaccine·Joeli A BrinkmanW Martin Kast
Mar 16, 2007·Journal of Translational Medicine·Christian AdamRalph Mocikat
Sep 26, 2007·Journal of Immunotherapy·Anke Kretz-RommelKatherine S Bowdish
Dec 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Theron S JohnsonAlexander H Enk
Feb 17, 2009·Expert Opinion on Biological Therapy·Yu-mei Wen
Nov 26, 2010·Journal of Medical Virology·Xin YaoYu-mei Wen
Dec 1, 2011·Journal of Internal Medicine·C TrumpfhellerR M Steinman
Dec 6, 2012·Molecular Pharmaceutics·Katharina WelserAlethea B Tabor
Dec 12, 2012·Kidney International·Ya WangStephen I Alexander
Aug 15, 2013·BMC Immunology·Jun CaoJian Yan
Aug 30, 2014·Journal of Pharmaceutical and Biomedical Analysis·Arvind K JainVicky L Kett
Citations
Apr 30, 2016·Journal of Viral Hepatitis·Y WangJ Y Yu
Oct 15, 2020·Signal Transduction and Targeted Therapy·Yetian DongFangfang Zhou
Sep 8, 2020·Angewandte Chemie·Christoph O FranckLjiljana Fruk
Oct 13, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Chen CuiLixiong Zhang