Design, fabrication and applications of tetrahedral DNA nanostructure-based multifunctional complexes in drug delivery and biomedical treatment

Nature Protocols
Tao ZhangYunfeng Lin

Abstract

Although organic nanomaterials and inorganic nanoparticles possess inherent flexibility, facilitating functional modification, increased intracellular uptake and controllable drug release, their underlying cytotoxicity and lack of specificity still cause safety concerns. Owing to their merits, which include natural biocompatibility, structural stability, unsurpassed programmability, ease of internalization and editable functionality, tetrahedral DNA nanostructures show promising potential as an alternative vehicle for drug delivery and biomedical treatment. Here, we describe the design, fabrication, purification, characterization and potential biomedical applications of a self-assembling tetrahedral DNA nanostructure (TDN)-based multifunctional delivery system. First, relying on Watson-Crick base pairing, four single DNA strands form a simple and typical pyramid structure via one hybridization step. Then, the protocol details four different modification approaches, including replacing a short sequence of a single DNA strand by an antisense peptide nucleic acid, appending an aptamer to the vertex, direct incubation with small-molecular-weight drugs such as paclitaxel and wogonin and coating with protective agents such as cationi...Continue Reading

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Citations

Mar 4, 2021·ACS Applied Materials & Interfaces·Qirong LiYunfeng Lin
Sep 26, 2020·ACS Applied Materials & Interfaces·Tao ZhangYunfeng Lin
Oct 20, 2021·ACS Applied Materials & Interfaces·Shaojingya GaoYunfeng Lin

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Methods Mentioned

BETA
transfection
biosensing
electrophoresis
atomic
dynamic light scattering
fluorescence
flow cytometry
biosensor
fluorescence spectroscopy
AFM

Software Mentioned

CanDo
One
FlowJo
- Analyzer
Cadnano
Tiamat
InCyte
Quantity

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