Design of a multi-epitope-based vaccine targeting M-protein of SARS-CoV2: an immunoinformatics approach.

Journal of Biomolecular Structure & Dynamics
Vijaya Sai AyyagariS Krupanidhi

Abstract

In the present study, one of the targets present on the envelopes of coronaviruses, membrane glycoprotein (M) was chosen for the design of a multi-epitope vaccine by Immunoinformatics approach. The B-cell and T-cell epitopes used for the construction of vaccine were antigenic, nonallergic and nontoxic. An adjuvant, β-defensin and PADRE sequence were included at the N-terminal end of the vaccine. All the epitopes were joined by linkers for decreasing the junctional immunogenicity. Various physicochemical parameters of the vaccine were evaluated. Secondary and tertiary structures were predicted for the vaccine construct. The tertiary structure was further refined, and various parameters related to the refinement of the protein structure were validated by using different tools. Humoral immunity induced by B-cells relies upon the identification of antigenic determinants on the surface of the vaccine construct. In this regard, the vaccine construct was found to consist of several B-cell epitopes in its three-dimensional conformation. Molecular docking of the vaccine was carried out with TLR-3 receptor to study their binding and its strength. Further, protein-protein interactions in the docked complex were visualized using LigPlot+. ...Continue Reading

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Citations

Feb 10, 2021·Advanced Drug Delivery Reviews·Woochang HwangNamshik Han
Apr 27, 2021·Informatics in Medicine Unlocked·Md Tahsin KhanMohammad A Halim
May 12, 2021·Journal, Genetic Engineering & Biotechnology·Harish Babu KollaVijaya Sai Ayyagari

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Datasets Mentioned

BETA
MT434760
MT292577
MT339041

Methods Mentioned

BETA
X-ray
NMR

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