Design of a Prediction Model for the Differentiation of Pasteurized Milk from Heated ESL Milk by Peptide Profiling

Proteomics
Sevim Dalabasmaz, Monika Pischetsrieder

Abstract

This study designs a prediction model to differentiate pasteurized milk from heated extended shelf life (ESL) milk based on milk peptides. For this purpose, quantitative peptide profiles of a training set of commercial samples including pasteurized (n = 20), pasteurized-ESL (n = 13), and heated-ESL (n = 16) milk are recorded by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Seven peptides are selected as putative markers, and cutoff levels and performance measures of each marker are defined by receiver operating characteristic (ROC) analysis. The accuracy of these peptides in the training set range between 71% and 90%. A prediction model is established based on the combined cutoff levels and evaluated by an independent blind test set. The processing method of 19 out of 20 unknown milk samples is predicted correctly achieving 95% accuracy. Five peptides of the prediction model are identified as αS1 -casein182-199 (m/z 2014.0), αS1 -casein180-199 (m/z 2216.1), αS1 -casein1-24 (m/z 2910.6), β-casein108-125 (m/z 2126.0), and β-casein106-125 (m/z 2391.2) indicating thermal release and the action of plasmin and cathepsins. Thus, the present study demonstrates that the milk peptide profile...Continue Reading

References

Oct 1, 1993·Journal of Dairy Science·H E Swaisgood
Aug 16, 2001·The Journal of Dairy Research·M G HayesA L Kelly
Oct 17, 2002·Journal of Agricultural and Food Chemistry·S Suzanne Nielsen
Dec 21, 2006·CJEM·Jerome FanAndrew Worster
Jun 3, 2009·The Psychiatric Clinics of North America·Jerald Belitz, Robert A Bailey
Jul 19, 2012·Proteomics·Juliana Regina BarreiroMarcos Veiga dos Santos
Aug 31, 2012·Journal of Agricultural and Food Chemistry·Cosima Damiana CalvanoCarlo Zambonin
Sep 14, 2012·Journal of Mass Spectrometry : JMS·Cosima Damiana CalvanoCarlo Giorgio Zambonin
Mar 13, 2013·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·N T GrabowskiG Klein
May 4, 2013·Journal of Proteomics·Rozaihan MansorP David Eckersall
Nov 20, 2013·Journal of Proteome Research·Florian BaumMonika Pischetsrieder
Dec 19, 2013·Journal of Agricultural and Food Chemistry·David C DallasJ Bruce German
Oct 21, 2014·Journal of Agricultural and Food Chemistry·Jasmin MeltretterMonika Pischetsrieder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved