Design of a ruthenium-cytochrome c derivative to measure electron transfer to the radical cation and oxyferryl heme in cytochrome c peroxidase.

Biochemistry
K WangF Millett

Abstract

A new ruthenium-labeled cytochrome c derivative was designed to measure the actual rate of electron transfer to the Trp-191 radical cation and the oxyferryl heme in cytochrome c peroxidase compound I {CMPI(FeIV = O,R.+)}. The H39C,C102T variant of yeast iso-1-cytochrome c was labeled at the single cysteine residue with a tris (bipyridyl)ruthenium(II) reagent to form Ru-39-Cc. This derivative has the same reactivity with CMPI as native yCc measured by stopped-flow spectroscopy, indicating that the ruthenium group does not interfere with the interaction between the two proteins. Laser excitation of the 1:1 Ru-39-Cc-CMPI complex in low ionic strength buffer (2 mM phosphate, pH 7) resulted in electron transfer from RuII* to heme c FeIII with a rate constant of 5 x 10(5) s-1, followed by electron transfer from heme c Fe II to the Trp-191 indolyl radical cation in CMPI(FeIV = O,R*+) with a rate constant of k(eta) = 2 x 10(6) s-1. A subsequent laser flash led to electron transfer from heme c to the oxyferryl heme in CMPII-(FeIV = O,R) with a rate constant of k(etb) = 5000 s-1. The location of the binding domain was determined using a series of surface charge mutants of CcP. The mutations D34N, E290N, and A193F each decreased the value...Continue Reading

References

Oct 27, 1976·Journal of the American Chemical Society·W L Purcell, J E Erman
Jun 1, 1995·Journal of Bioenergetics and Biomembranes·M J BjerrumJ R Winkler

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Citations

Apr 16, 2004·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Tamal Sengupta, Samita Basu
Jun 5, 2002·Biochimica Et Biophysica Acta·James E Erman, Lidia B Vitello
Dec 17, 2008·Biochemistry·Anna-Maria A Hays PutnamDavid B Goodin
Aug 19, 2007·Chemical Communications : Chem Comm·Joshua R PetersonPall Thordarson
Dec 14, 2012·PLoS Computational Biology·Alexander N Volkov, Nico A J van Nuland
Nov 17, 2011·European Biophysics Journal : EBJ·Irene Díaz-MorenoMiguel A De la Rosa
Nov 21, 2014·Journal of the Royal Society, Interface·Marian BreuerJulea N Butt
Oct 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Seong A Kang, Brian R Crane
Jan 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Georges ChreifiThomas L Poulos
Aug 9, 2011·Biochimica Et Biophysica Acta·Alexander N VolkovJonathan A R Worrall
Dec 19, 2003·Biochemical and Biophysical Research Communications·Alina OlteanuGary J Pielak
May 7, 2015·Nature Communications·Karen Van de WaterAlexander N Volkov
Dec 22, 2009·Chemical Communications : Chem Comm·Vanesa Fernández-MoreiraMichael P Coogan
Jan 26, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Niall J O'Reilly, Edmond Magner
Apr 10, 1999·The Journal of Biological Chemistry·G W PettigrewJ J Moura
Jun 9, 2005·Chemical Reviews·Konrad SzaciłowskiGrazyna Stochel
Jul 9, 2021·The Journal of Physical Chemistry. B·Xuyan RuDavid N Beratan
Oct 12, 2019·Journal of the American Chemical Society·Estella F YeeBrian R Crane
Aug 26, 2017·Journal of Chemical Information and Modeling·Scott A HollingsworthThomas L Poulos

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