Design of a side-view particle imaging velocimetry flow system for cell-substrate adhesion studies

Journal of Biomechanical Engineering
Jordan Leyton-MangeCheng Dong

Abstract

Experimental models that mimic the flow conditions in microcapillaries have suggested that the local shear stresses and shear rates can mediate tumor cell and leukocyte arrest on the endothelium and subsequent sustained adhesion. However, further investigation has been limited by the lack of experimental models that allow quantitative measurement of the hydrodynamic environment over adherent cells. The purpose of this study was to develop a system capable of acquiring quantitative flow profiles over adherent cells. By combining the techniques of side-view imaging and particle image velocimetry (PIV), an in vitro model was constructed that is capable of obtaining quantitative flow data over cells adhering to the endothelium. The velocity over an adherent leukocyte was measured and the shear rate was calculated under low and high upstream wall shear. The microcapillary channel was modeled using computational fluid dynamics (CFD) and the calculated velocity profiles over cells under the low and high shear rates were compared to experimental results. The drag force applied to each cell by the fluid was then computed. This system provides a means for future study of the forces underlying adhesion by permitting characterization of th...Continue Reading

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Citations

Mar 5, 2009·Annals of Biomedical Engineering·Dennis DischerSheldon Weinbaum
Mar 25, 2009·ACS Nano·Kevin SealeJohn Wikswo
Sep 27, 2007·Lab on a Chip·Xiaole MaoTony Jun Huang
Jun 9, 2010·Journal of Biomechanical Engineering·Sean S KohlesDerek C Tretheway
Nov 20, 2008·Journal of Microscopy·K T SealeJ P Wikswo
May 20, 2009·Biophysical Journal·John E Pickard, Klaus Ley
Nov 4, 2010·Biophysical Journal·Andrew D BaikX Edward Guo
Jul 19, 2011·Microvascular Research·Balabhaskar PrabhakarpandianMohammad F Kiani
Jul 10, 2012·Biophysical Journal·Damir B Khismatullin, George A Truskey
Jan 26, 2018·Scientific Reports·Kellie BeickerRichard Superfine
Dec 30, 2014·Cellular and Molecular Bioengineering·Alexandre J S RibeiroKris Noel Dahl
May 1, 2008·Experimental Biology and Medicine·Kweku A Addae-Mensah, John P Wikswo

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