PMID: 3768430Oct 31, 1986Paper

Design of an indicator of intracellular free Na+ concentration using 19F-NMR

Biochimica Et Biophysica Acta
G A SmithJ C Metcalfe

Abstract

The development is described of an Na+ chelator with appropriate properties for an indicator of intracellular free Na+ concentration ([Na+]i). The new indicator, FCryp-1, is a tribenzo derivative of the parent (2:2:1) cryptand structure, incorporating the same F-substituted dibenzo 19F-NMR reporter group as the free [Ca2+] indicator, 5FBAPTA (Smith, G.A., Hesketh, T.R., Metcalfe, J.C., Feeney, J. and Morris, P.G. (1983) Proc. Natl. Acad. Sci., USA 80, 7178-7182). FCryp-1 has appropriate affinity for Na+ (KNa = 10(1.3) M-1) and selectivity over other intracellular cations (KK; KCa; K Mg less than 10(-1) M(-1)) for a [Na]i indicator. There is an 19F-NMR chemical shift of 2.00 ppm between free FCryp-1 and the Na-FCryp-1 complex which provides a direct read out of free [Na+]. FCryp-1 carries four carboxylate groups to confer aqueous solubility which can be esterified with acetoxymethyl groups to render the indicator membrane permeant. Experiments on pig lymphocytes loaded with FCryp-1 gave an indicated [Na+]i of 13.8 +/- 1.8 mM (n = 4). The FCryp-1 structure can also be readily modified to provide fluorescent [Na+]i indicators.

References

Apr 20, 1978·The Journal of Membrane Biology·L G PalmerM M Civan
Mar 30, 1973·Annals of the New York Academy of Sciences·H J Berendsen, H T Edzes
Jul 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·F W Cope
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Citations

Sep 1, 1994·Progress in Neurobiology·R A Kauppinen, S R Williams
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H L KirschenlohrG A Smith
Apr 2, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Jian-Xin YuRalph P Mason
Jun 1, 1989·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M C Malet-Martino, R Martino
Sep 30, 2004·Bioorganic & Medicinal Chemistry Letters·Vladimir V MartinKyle R Gee
Mar 31, 1994·Biochimica Et Biophysica Acta·T IvanicsM Osbakken
Dec 1, 1990·Analytical Biochemistry·P LundbergH J Vogel
Aug 7, 2018·Chemical Reviews·Zhenchuang XuYanchuan Zhao

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