Design of biocompatible immobilized Candida rugosa lipase with potential application in food industry

Journal of the Science of Food and Agriculture
Jovana Trbojević IvićNenad Milosavić

Abstract

Biocatalysts are a promising alternative for the production of natural flavor compounds. Candida rugosa lipase (CRL) is a particularly important biocatalyst owing to its remarkable efficiency in both hydrolysis and synthesis. However, additional stabilization is necessary for successful industrial implementation. This study presents an easy and time-saving method for immobilizing this valuable enzyme on hydroxyapatite (HAP), a biomaterial with high protein-binding capacity. Targeted immobilized CRL was obtained in high yield of ≥98%. Significant lipase stabilization was observed upon immobilization: at 60 °C, immobilized lipase (HAP-CRL) retained almost unchanged activity after 3 h, while free CRL lost 50% of its initial activity after only 30 min. The same trend was observed with tested organic solvents. Methanol and hexane had the most pronounced effect: after 3 h, only HAP-CRL was stable and active, while CRL was completely inactivated. The practical value of the prepared catalyst was tested in the synthesis of the aroma ester methyl acetate in hexane. Reaction yields were 2.6 and 52.5% for CRL and HAP-CRL respectively. This research has successfully combined an industrially prominent biocatalyst, CRL, and a biocompatible, e...Continue Reading

References

Aug 28, 1998·Chemistry and Physics of Lipids·J PleissR D Schmid
Apr 29, 2008·Biomaterials·David F Williams
Jan 3, 2012·Bioresource Technology·Aleksandra DimitrijevićNenad Milosavić
Mar 1, 2012·Journal of Biotechnology·Zhang-Qun DuanDe-Hua Liu
Mar 23, 2013·Chemical Society Reviews·Maurice C R FranssenJohan P M Sanders

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Citations

Oct 11, 2016·Food Chemistry·Jovana Trbojević IvićDušan Veličković
Jun 6, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Cheng Huan LiuJei Fu Shaw
May 20, 2020·Critical Reviews in Food Science and Nutrition·Ana Letícia Silva Coelho, Ravely Casarotti Orlandelli
Jul 26, 2019·Applied Biochemistry and Biotechnology·Thamara C CoutinhoCristiane S Farinas
Apr 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·María Molina-GutiérrezAlicia Prieto
Mar 6, 2021·International Journal of Biological Macromolecules·F Rafiee, M Rezaee
Feb 12, 2020·International Journal of Biological Macromolecules·Thamara C CoutinhoCristiane S Farinas

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