Design of countercurrent separation of Ginkgo biloba terpene lactones by nuclear magnetic resonance.

Journal of Chromatography. a
Feng QiuGuido F Pauli

Abstract

Terpene lactones such as bilobalide, ginkgolides A, B, C, and J are major bioactive compounds of Ginkgo biloba L. Purification of these compounds is tedious due to their similar chemical properties. For the purpose of developing an effective and efficient method for both analytical and preparative separation of terpene lactones in G. biloba, an innovative orthogonality-enhanced high-speed countercurrent chromatography (HSCCC) method was established. Taking advantage of quantitative (1)H NMR (qHNMR) methodology, partition coefficients (K) of individual terpene lactones were calculated directly from crude G. biloba leaf extract, using their H-12 signals as distinguishing feature. The partitioning experiment assisted the design of a two dimensional (2D) HSCCC procedure using a pair of orthogonal HSCCC solvent systems (SSs), ChMWat +4 and HEMSoWat +3/0.05%. It was surprising that the resolution of ginkgolides A and B was improved by 25% in the HEMWat +3 SS modified with 0.5% DMSO. Consequently, all five terpene lactones could be well separated with qHNMR purity>95% from G. biloba leaf extract. The separation was further evaluated by offline qHNMR analysis of HSCCC fractions associated with Gaussian curve fitting. The results showed...Continue Reading

References

Nov 7, 2001·Journal of Chromatography. a·V B Di Marco, G G Bombi
Nov 14, 2001·Phytochemical Analysis : PCA·J L WolfenderK Hostettmann
Feb 11, 2003·Chemical & Pharmaceutical Bulletin·Young Hae ChoiRobert Verpoorte
Jun 10, 2004·Journal of Agricultural and Food Chemistry·Chia-Ying LiTian-Shung Wu
Oct 27, 2004·Phytochemistry·Stanislav JaraczKoji Nakanishi
Feb 1, 2005·Journal of Natural Products·Guido F PauliDavid C Lankin
Jul 5, 2005·Bioorganic & Medicinal Chemistry·Teris A van Beek
Feb 15, 2007·Analytical Chemistry·J Brent Friesen, Guido F Pauli
Sep 1, 2007·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Po-Chuen ChanPeter P Fu
Apr 1, 1995·Talanta·S Walsh, D Diamond
Dec 26, 2008·Journal of Chromatography. a·Frank L WagenaarPhilip A Searle
Nov 12, 2010·Journal of Chromatography. a·Andrew R Johnson, Mark F Vitha
Apr 8, 2011·Journal of Chromatography. a·Svetlana IgnatovaIan Sutherland
May 4, 2011·Journal of Chromatography. a·Walter D Conway
Feb 11, 2012·Journal of Natural Products·Feng QiuGuido F Pauli

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Citations

Dec 19, 2012·Journal of Natural Products·Shi-Hui DongShao-Nong Chen
Nov 14, 2013·BioMed Research International·Daniely Cornélio FavarinAlexandre de Paula Rogerio
Jun 6, 2014·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Krystyna Skalicka-Woźniak, Ian Garrard
Aug 24, 2013·Fitoterapia·Shi-Hui DongShao-Nong Chen
May 24, 2014·Journal of Pharmaceutical and Biomedical Analysis·Rubén M MaggioTeodoro S Kaufman
Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Feb 4, 2014·Current Opinion in Biotechnology·Charlotte SimmlerGuido F Pauli
Dec 30, 2014·Journal of Chromatography. a·J Brent FriesenGuido F Pauli
Oct 24, 2019·Journal of Separation Science·Honghong LiHaiyan Wang
Aug 12, 2017·Topics in Current Chemistry·Leslie BrownKenneth R Seddon
Jul 8, 2020·Journal of Molecular Modeling·Xusheng HuangZhaojun Xian
Jul 15, 2015·Analytical Chemistry·Guido F PauliJ Brent Friesen

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