Design of Modular Protein Tags for Orthogonal Covalent Bond Formation at Specific DNA Sequences

Journal of the American Chemical Society
Thang Minh NguyenTakashi Morii

Abstract

Simultaneous formation of specific covalent linkages at nucleotides in given DNA sequences demand distinct orthogonal reactivity of DNA modification agents. Such highly specific reactions require well-balanced reactivity and affinity of the DNA modification agents. Conjugation of a sequence-specific DNA binding zinc finger protein and a self-ligating protein tag provides a modular adaptor that expedites formation of a covalent bond between the protein tag and a substrate-modified nucleotide at a specific DNA sequence. The modular adaptor stably locates a protein of interest fused to it at the target position on DNA scaffold in its functional form. Modular adaptors with orthogonal selectivity and fast reaction kinetics to specific DNA sequences enable site-specific location of different protein molecules simultaneously. Three different modular adaptors consisting of zinc finger proteins with distinct DNA sequence specificities and self-ligating protein tags with different substrate specificities achieved orthogonal covalent bond formation at respective sequences on the same DNA scaffold with an overall coassembly yield over 90%. Application of this unique set of orthogonal modular adaptors enabled construction of a cascade react...Continue Reading

References

Jul 1, 1979·Journal of Bacteriology·D K Shamanna, K E Sanderson
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J S Kim, C O Pabo
Dec 26, 2001·Journal of the American Chemical Society·J M GarganoW J Lees
Dec 7, 2002·Nature Biotechnology·Antje KepplerKai Johnsson
Jan 24, 2003·Nature·Nadrian C Seeman
Jun 5, 2004·Nucleic Acids Research·Stephen E Halford, John F Marko
Mar 17, 2006·Nature·Paul W K Rothemund
May 23, 2006·Current Opinion in Biotechnology·Thomas W Jeffries
Dec 21, 2006·Accounts of Chemical Research·Toshikazu Bando, Hiroshi Sugiyama
Jan 11, 2007·Trends in Biochemical Sciences·Roland GamsjaegerJoel P Mackay
Feb 1, 2007·Journal of the American Chemical Society·Vijay M KrishnamurthyGeorge M Whitesides
Nov 6, 2007·Trends in Biochemical Sciences·Joel P MackayJacqueline M Matthews
Feb 23, 2008·Chemistry & Biology·Arnaud GautierKai Johnsson
Jun 7, 2008·ACS Chemical Biology·Georgyi V LosKeith V Wood
Feb 7, 2009·Chemical Reviews·G SchreiberH-X Zhou
Mar 18, 2009·Biochemical Society Transactions·Stephen E Halford
Apr 8, 2009·Nature Nanotechnology·Ofer I WilnerItamar Willner
Jun 6, 2009·Nature Nanotechnology·Mark Freeman, Wayne Hiebert
Jul 3, 2009·Applied Microbiology and Biotechnology·Akinori MatsushikaShigeki Sawayama
Jan 22, 2010·Angewandte Chemie·Christof M Niemeyer
Oct 30, 2010·Current Opinion in Biotechnology·Marlon J Hinner, Kai Johnsson
Oct 30, 2010·Angewandte Chemie·Barbara SaccàChristof M Niemeyer
Nov 8, 2011·Nature Nanotechnology·Andre V PinheiroHao Yan
Jan 31, 2012·Angewandte Chemie·Eiji NakataTakashi Morii
Mar 15, 2012·Journal of the American Chemical Society·Jinglin FuHao Yan
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marcel GeertzSebastian J Maerkl
Nov 5, 2013·Methods : a Companion to Methods in Enzymology·Tien Anh NgoTakashi Morii
Jan 7, 2014·Nature Nanotechnology
Jan 18, 2014·ACS Chemical Biology·Kathrin Lang, Jason W Chin
Aug 1, 2013·Israel Journal of Chemistry·Elissa M HobertAlanna Schepartz
May 12, 2015·Bioconjugate Chemistry·Yuhe R YangHao Yan
May 15, 2015·Bioconjugate Chemistry·Christopher G EnglandWeibo Cai
Feb 18, 2016·Journal of the American Chemical Society·Tien Anh NgoTakashi Morii
Feb 27, 2016·Angewandte Chemie·Sandra SagredoFernando de la Cruz
Mar 2, 2016·Chembiochem : a European Journal of Chemical Biology·Arun Richard ChandrasekaranXing Wang
Mar 15, 2016·Chembiochem : a European Journal of Chemical Biology·Katja J KoßmannChristof M Niemeyer
Mar 24, 2016·Nature Chemistry·Ian WheeldonMatthew Sigman
May 6, 2016·Nature Nanotechnology·Andreas KüchlerPeter Walde
Feb 6, 2017·Chembiochem : a European Journal of Chemical Biology·Arivazhagan RajendranTakashi Morii
Jan 1, 2016·Nanomaterials·Veikko LinkoMauri A Kostiainen

❮ Previous
Next ❯

Citations

Jan 18, 2018·Angewandte Chemie·Tatsuki KurokawaYasuo Mori
Jan 5, 2019·Chemistry : a European Journal·Teresa BurgahnChristof M Niemeyer
Oct 3, 2019·Chemical Communications : Chem Comm·Tien Anh NgoTakashi Morii
Apr 3, 2020·Chemical Communications : Chem Comm·Samuel LimDouglas S Clark
Apr 6, 2020·Topics in Current Chemistry·Jinglin FuTing Zhang
Mar 18, 2021·Nature Communications·Yang-Ha LeeCheol-Min Park
Apr 20, 2021·Chemical Communications : Chem Comm·Peng LinTakashi Morii
Jul 13, 2021·Frontiers in Chemistry·Peng LinTakashi Morii
Feb 5, 2019·Chemical Reviews·Mikael Madsen, Kurt V Gothelf
Jun 22, 2018·Journal of the American Chemical Society·Yahong ChenChaoyong James Yang
Aug 30, 2018·ACS Applied Materials & Interfaces·Chao XingHuang-Hao Yang
Oct 9, 2021·Chemical Communications : Chem Comm·Peng LinTakashi Morii

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.