Design of Surfactant Protein B Peptide Mimics Based on the Saposin Fold for Synthetic Lung Surfactants

Biomedicine Hub
F J WaltherA J Waring

Abstract

Surfactant protein (SP)-B is a 79-residue polypeptide crucial for the biophysical and physiological function of endogenous lung surfactant. SP-B is a member of the Saposin or Saposin-like proteins (SAPLIP) family of proteins that share an overall three-dimensional folding pattern based on secondary structures and disulfide connectivity and exhibit a wide diversity of biological functions. Here we review the synthesis, molecular biophysics and activity of synthetic analogs of Saposin proteins designed to mimic those interactions of the parent proteins with lipids that enhance interfacial activity. Saposin proteins generally interact with target lipids as either monomers or multimers via well-defined amphipathic helices, flexible hinge domains, and insertion sequences. Based on the known 3D-structural motif for the Saposin family, we show how bioengineering techniques may be used to develop minimal peptide constructs that maintain desirable structural properties and activities in biomedical applications. One important application is the molecular design, synthesis and activity of Saposin mimics based on the SP-B structure. Synthetic lung surfactants containing active SP-B analogs may be potentially useful in treating diseases of ...Continue Reading

Citations

Mar 1, 2019·Expert Review of Respiratory Medicine·Frans J WaltherAlan J Waring
Aug 11, 2019·International Journal of Molecular Sciences·Nicholas A S RobichaudValerie Booth
Apr 12, 2021·European Journal of Pediatrics·Jagdev SinghHiran Selvadurai
Oct 14, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Laurianne Gravel-TattaAntonella Badia
Mar 2, 2021·Protein and Peptide Letters·Yi-Shan TangFu Chen

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Methods Mentioned

BETA
NMR
X-ray
atomic force microscopy
surface plasmon resonance
lavage

Software Mentioned

TASSER
Infasurf®
TM
PASTA
align

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