Apr 27, 2016

Membrane tension is a key determinant of bud morphology in clathrin-mediated endocytosis

BioRxiv : the Preprint Server for Biology
Julian E HassingerPadmini Rangamani

Abstract

In clathrin-mediated endocytosis (CME), clathrin and various adaptor proteins coat a patch of the plasma membrane, which is reshaped to form a budded vesicle. Experimental studies have demonstrated that elevated membrane tension can inhibit bud formation by a clathrin coat. In this study, we investigate the impact of membrane tension on the mechanics of membrane budding by simulating clathrin coats that either grow in area or progressively induce greater curvature. At low membrane tension, progressively increasing the area of a curvature-generating coat causes the membrane to smoothly evolve from a flat to budded morphology, whereas the membrane remains essentially flat at high membrane tensions. Interestingly, at physiologically relevant, intermediate membrane tensions, the shape evolution of the membrane undergoes a snapthrough instability in which increasing coat area causes the membrane to "snap" from an open, U-shaped bud to a closed, Ω-shaped bud. This instability is accompanied by a large energy barrier, which could cause a developing endocytic pit to stall if the binding energy of additional coat is insufficient to overcome this barrier. Similar results were found for a coat of constant area in which the spontaneous cur...Continue Reading

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Mentioned in this Paper

Research in General
Study
Muscle Rigidity
Biochemical Pathway
Membrane Budding
Tissue Membrane
Theoretical Model
COPA
Decompression Sickness
Membrane

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