Design, recombinant expression, and antibacterial activity of the cecropins-melittin hybrid antimicrobial peptides.

Current Microbiology
Yu CaoDai Rong Qiao

Abstract

In order to evaluate their antibacterial activities and toxicities, the cecropins-melittin hybrid antimicrobial peptide, CA(1-7)-M(4-11) (CAM) and CB(1-7)-M(4-11) (CBM), were designed by APD2 database. The recombinant hybrid antimicrobial peptides were successfully expressed and purified in Pichia pastoris. Antimicrobial activity assay showed that both of the two hybrid antimicrobial peptides had strong antibacterial abilities against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis, Bacillus thuringiensis, and Salmonella derby. The potency of CAM and CBM to E. coli 25922 were 0.862 and 0.849, respectively, slightly lower than Amp's 0.957. The hemolytic assays indicated CAM and CBM had no hemolytic in vivo and in vitro, and so they had a good application prospect.

References

Jan 1, 1990·Annual Review of Biochemistry·C L Bevins, M Zasloff
Jan 1, 1995·Annual Review of Immunology·H G Boman
Feb 8, 1997·Lancet·R E Hancock
Jan 30, 2002·FEMS Microbiology Letters·Robert E W Hancock, Annett Rozek
Apr 12, 2002·Current Pharmaceutical Design·Yechiel Shai
Mar 5, 2003·Pharmacological Reviews·Michael R Yeaman, Nannette Y Yount
Mar 17, 2004·Peptides·Jon-Paul S Powers, Robert E W Hancock
Mar 12, 2005·Journal of the American Academy of Dermatology·Arash Izadpanah, Richard L Gallo
Jun 4, 2005·Journal of the American Academy of Dermatology·Ginat W Mirowski
May 24, 2006·Biochimica Et Biophysica Acta·Ulrich H N DürrAyyalusamy Ramamoorthy
Sep 19, 2006·Biochimica Et Biophysica Acta·Vishnu DhopleAyyalusamy Ramamoorthy
Apr 17, 2007·Biotechnology and Applied Biochemistry·Aaron B Ingham, Robert J Moore
Jun 27, 2009·The Journal of Microbiology·Soon-ja KimJoong-Kook Choi

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Citations

May 23, 2012·International Journal of Peptides·C PolancoJ A Castanon-Gonzalez
Nov 28, 2013·Journal of Industrial Microbiology & Biotechnology·Xing-Jun FengZhong-Qiu Li
Jul 18, 2015·Pharmaceuticals·Biljana Mojsoska, Håvard Jenssen
Dec 21, 2010·Journal of Peptide Science : an Official Publication of the European Peptide Society·Xiuhong LiuRen Lai
Oct 14, 2014·PloS One·Chih-Yu HuangHueih-Min Chen
Jan 11, 2017·Scientific Reports·Shengyue JiHongfu Zhang
Nov 15, 2011·Critical Reviews in Biotechnology·Mukesh PasupuletiMartin Malmsten
Feb 14, 2019·The Journal of Antibiotics·A Seher Birteksoz-TanCagla Bozkurt-Guzel
Feb 27, 2020·International Journal of Molecular Sciences·Yang YangWenping Gan
May 18, 2020·Applied Microbiology and Biotechnology·S A NavarroMiriam C Chalón

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