Abstract
Immunotherapeutic approaches are investigated for treatment of neurodegenerative diseases of the Alzheimer's dementia (AD) type. The identification of a beta-amyloid-plaque specific epitope, Abeta(4-10) (4FRHDSGY10), recognized by therapeutically active antibodies from transgenic AD mice could provide the basis for the development of AD vaccines. Here we report on the synthesis, structural and immuno-analytical characterization of bioconjugates comprising the beta-amyloid(4-10) epitope as new vaccine lead structures against Alzheimer's disease. To produce antigenic bioconjugates, potential immunogens, the epitope peptide elongated by a cysteine residue or a cysteinyl-pentaglycine hexapeptide unit either at the N- or C-terminus was attached via a thioether bond to synthetic oligopeptide carriers, such as oligotuftsin derivatives, sequential oligopeptide carrier, or lysine dendrimer. The antigenic properties of these constructs were determined by enzyme-linked immunosorbent assay (ELISA) using an anti-Abeta(1-17) monoclonal antibody. Our results indicate that the major factors which influence the antibody binding of the Abeta(4-10) epitope are (i) the epitope topology and (ii) the presence of a spacer moiety between the carrier a...Continue Reading
References
Mar 1, 1989·Analytical Biochemistry·F A Robey, R L Fields
May 10, 1996·Journal of Immunological Methods·C BoecklerF Schuber
Feb 24, 2001·Annals of the New York Academy of Sciences·D J Selkoe
Jul 20, 2002·Science·John Hardy, Dennis J Selkoe
Oct 16, 2002·Nature Medicine·J McLaurinP St George-Hyslop
Nov 20, 2003·Bioconjugate Chemistry·Gábor MezöFerenc Hudecz
Mar 30, 2004·Biopolymers·Gábor MezöFerenc Hudecz
Aug 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·David S GelinasJoanne McLaurin
Oct 23, 2004·Biopolymers·Marilena ManeaMichael Przybylski
Jan 7, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Gábor MezöFerenc Hudecz
Jul 21, 2005·Bioconjugate Chemistry·Marilena ManeaGábor Mezõ
Oct 26, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Gábor MezöFerenc Hudecz
Dec 3, 2005·European Journal of Mass Spectrometry·Xiaodan TianMichael Przybylski
Dec 13, 2005·Autoimmunity Reviews·Valeria Geylis, Michael Steinitz
Feb 24, 2006·Biopolymers·Dimitrios KrikorianVassilios Tsikaris
May 31, 2006·Cell Biology International·O LángL Kohidai
Citations
Nov 17, 2009·Archives of Virology·Zhenhua ZhengNingshao Xia
Mar 26, 2009·Journal of Peptide Science : an Official Publication of the European Peptide Society·Kata HorvátiSzilvia Bosze
Jan 13, 2018·Journal of Peptide Science : an Official Publication of the European Peptide Society·Raluca ȘtefănescuMichael Przybylski
Oct 31, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martin CornilletLeonor Nogueira
Jan 27, 2019·International Journal of Molecular Sciences·Vo Van Giau, Seong Soo A An