Design, synthesis and evaluation of anticancer activity of ruthenium (II) polypyridyl complexes

Journal of Inorganic Biochemistry
Bing TangYun-Jun Liu

Abstract

A new ligand PFPIP (PFPIP=2-(2,3,4,5,6-pentafluorophenyl)[4,5-f]imadazo [1,10]phenanthroline) and its four ruthenium(II) polypyridyl complexes [Ru(NN)2(PFPIP)](ClO4)2(NN=dmb: 4,4'-dimethyl-2,2'-bipyridine, 1; bpy: 2,2'-bipyridine, 2; phen: 1,10-phenanthroline, 3; dmp: 2,9-dimethyl-1,10-phenanthroline, 4) were synthesized and characterized by elemental analysis, IR,1H NMR,13C NMR and ESI-MS. The cytotoxic activity in vitro of the ligand and complexes toward BEL-7402, A549, HeLa, HepG2 and MG-63 cell lines was evaluated using MTT method (MTT=(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Complexes 1, 3 and 4 show moderate cytotoxic effect on the cell growth in BEL-7402 cells with IC50values of 32.1±0.9, 37.9±1.7 and 42.1±3.0μM, respectively. The apoptosis in BEL-7402 cell was investigated with AO/EB and Hoechst 33,258 staining methods. The autophagy in BEL-7402 cell induced by complexes was assayed using MDC staining cell nuclei. The cell invasion, reactive oxygen species (ROS), mitochondrial membrane potential, cell cycle arrest, cellular uptake, comet assay and wound healing were studied under a fluorescent microscope. The complexes can cause autophagy and inhibit the cell invasion, and increase the ROS levels ...Continue Reading

Citations

Mar 22, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Anja MolterFabian Mohr
Nov 28, 2017·Chemical Society Reviews·Fergus E PoyntonThorfinnur Gunnlaugsson
Dec 7, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mariana S de CamargoAlzir A Batista
Oct 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vaiyapuri Subbarayan PeriasamyMohammad Abdulkader Akbarsha

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