Design, Synthesis, and Use of Novel Photoaffinity Probes in Measuring the Serum Concentration of Glycogen Phosphorylase

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yuchao ZhangLiying Zhang

Abstract

A procedure to measure the serum concentration of glycogen phosphorylase during acute myocardial infarction is presented. This method was based on the synthesis of photoaffinity probes, and used the semiquantitative protein electrophoretic mobility shift technique. Three novel photoaffinity probes bearing different secondary tags were synthesized. Their potency was evaluated in an enzyme inhibition assay against rabbit muscle glycogen phosphorylase a (RMGPa). The inhibitory activity of probe 1 was only 100-fold less potent than the mother compound CP-320626. The photoaffinity labeling experiments were also performed, and a protein with molecular weight (MW) of about 90⁻100 kDa, which was consistent with the MW of GP, was clearly labeled by probe 1. A semiquantitative evaluation of the GP level in serum with probe 1 was also performed. The results showed that the protein band with a MW of about 90⁻100 kDa was tagged, and the concentration of the protein in serum was found to be between 25 and 50 ng/mL. Mass spectrometric analysis revealed that alpha-1,4 glucan phosphorylase (GPMM) was well-preserved in the bands.

References

Jan 20, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·E G KrauseA Wollenberger
Jul 1, 1996·Molecular and Cellular Biochemistry·E G KrauseB Puschendorf
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·W H MartinJ L Treadway
May 15, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·J Mair
Nov 4, 2004·Journal of the American Chemical Society·Elaine W S ChanShao Q Yao
Mar 18, 2008·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·David StejskalLudek Sprongl
Oct 1, 2008·Bioorganic & Medicinal Chemistry Letters·Michael B HarbutDoron C Greenbaum
Dec 30, 2011·Bioorganic & Medicinal Chemistry Letters·Liying ZhangHongbin Sun
Feb 17, 2015·Future Medicinal Chemistry·Ewan Smith, Ian Collins

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Methods Mentioned

BETA
coronary artery bypass
affinity purification
Assay
NMR
column chromatography
reversed-phase chromatography

Software Mentioned

Quantity One
GPMM
MaxQuant
GPBB

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