Designed diblock hairpin probes for the nonenzymatic and label-free detection of nucleic acid

Biosensors & Bioelectronics
Junlin WenShungui Zhou

Abstract

The detection of nucleic acid sequences is of great importance in a variety of fields. An ultrasensitive DNA sensing platform is constructed using elaborately designed diblock hairpin probes (DHPs) that are composed of hairpin and poly-adenine blocks. The introduction of an initiator DNA target triggers the catalytic assembly of probes DHP1, DHP2 and DHP3 to fabricate numerous poly-adenine-tailed branched DNA junctions, which significantly amplify the signal of the target-DNA-recognizing event without any enzyme. Coupled to a gold nanoparticle-based colorimetric assay, the amplified recognition signal can be quantitatively detected or visually read with the naked eye. The combination of the high-efficiency target-catalyzed DHP assembly and sensitive gold-based colorimetric assay offers an ultrasensitive detection of DNA with a detection limit of 0.1 pM and a dynamic range from 0.01 to 5 pM. The proposed sensing platform can discriminate even single-base mutations. Moreover, the sensing platform can be expanded to detect pollutant-degrading-bacteria-specific DNA sequences. The proposed sensing system should offer an alternative approach for the detection of nucleic acids in the fields of microbiology, biogeochemistry, and enviro...Continue Reading

References

Nov 28, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Xu ZhangJuewen Liu
Sep 21, 2013·Journal of Colloid and Interface Science·Hui JiangJuewen Liu

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Citations

Mar 1, 2018·Annual Review of Analytical Chemistry·Dekai YeChunhai Fan
Jun 3, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P MaffertH Abaibou
Apr 21, 2021·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Shuang ZhaoMing Chen
Oct 8, 2019·Analytical Chemistry·Xian ChenHuanghao Yang

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