Designed synthesis of Graphene @titania @mesoporous silica hybrid material as size-exclusive metal oxide affinity chromatography platform for selective enrichment of endogenous phosphopeptides

Talanta
Jizong YaoXiangming Zhang

Abstract

In this work, a novel size-exclusive metal oxide affinity chromatography (SE-MOAC) platform was built for phosphoproteome research. The operation for preparing graphene @titania @mesoporous silica nanohybrids (denoted as G@TiO2@mSiO2) was facile and easy to conduct by grafting titania nanoparticles on polydopamine (PD)-covered graphene, following a layer of mesoporous silica was coated on the outermost layer. The G@TiO2@mSiO2 nanohybrids exhibited high sensitivity with a low detection limit of 5 amol/μL (a total amount of 1 fmol) and high selectivity for phosphopeptides at a mass ratio of phosphopeptides to non-phosphopeptides (1:1000). The size-exclusive capability of the nanohybrids were also demonstrated by enriching the phosphopeptides from the mixture of Bovine Serum Albumin (BSA), α-casein, and β-casein digests with a high mass ratio (β-casein digests: α-casein: BSA, 1:500:500), which was attributed to the large surface area and ordered mesoporous channels. In addition, the G@TiO2@mSiO2 nanohybrids were employed to capture the endogenous phosphopeptides from human serum successfully.

References

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Jun 10, 2014·ACS Applied Materials & Interfaces·Mengyi WangXiangmin Zhang

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Citations

May 3, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Joanna HajdukZenon J Kokot
Jan 11, 2018·Analytical and Bioanalytical Chemistry·Giorgia La BarberaAldo Laganà
Jan 27, 2019·Analytical and Bioanalytical Chemistry·Adeela SaeedMuhammad Najam-Ul-Haq

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