Designer probiotic Lactobacillus plantarum expressing oxalate decarboxylase developed using group II intron degrades intestinal oxalate in hyperoxaluric rats

Microbiological Research
Eldho PaulSelvam Govindan Sadasivam

Abstract

Increased intestinal absorption of oxalate causes hyperoxaluria, a major risk factor for kidney stone disease. Intestinal colonization of recombinant probiotic bacteria expressing oxalate-degrading gene (OxdC) is an effective therapeutic option for recurrent calcium oxalate (CaOx) stone disease. Therefore, we aimed to develop food-grade probiotic L. plantarum secreting OxdC using lactococcal group II intron, Ll.LtrB and evaluate its oxalate degradation ability in vivo. Male Wistar albino rats were divided into four groups. The rats of group I received normal rat chow and drinking water. Groups II, III and IV rats received 5% potassium oxalate containing diet for 28 days. Groups III and IV rats received L. plantarum and food-grade recombinant L. plantarum respectively from 15 to 28 days. Biochemical parameters and crystalluria were analysed in 24 h urine samples. At the end of experimental period, rats were sacrificed; intestine and kidneys were dissected out for colonization studies and histopathological analysis. Herein, we found that the administration of recombinant probiotics significantly reduced the urinary oxalate, calcium, urea, and creatinine levels in rats of group IV compared to group II. Furthermore, colonization st...Continue Reading

Citations

May 14, 2020·Current Opinion in Nephrology and Hypertension·Elizabeth P KwendaBenjamin K Canales
Nov 26, 2019·3 Biotech·Monika Yadav, Pratyoosh Shukla
Jun 8, 2021·The Journal of Biological Chemistry·Anthony J PastoreAlexander Angerhofer
Jul 18, 2021·Advanced Drug Delivery Reviews·Adriana Mejía-PittaCesar de la Fuente-Nunez

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