Designing a binding interface for control of cancer cell adhesion via 3D topography and metabolic oligosaccharide engineering.

Biomaterials
Jian DuKevin J Yarema

Abstract

This study combines metabolic oligosaccharide engineering (MOE), a technology where the glycocalyx of living cells is endowed with chemical features not normally found in sugars, with custom-designed three-dimensional biomaterial substrates to enhance the adhesion of cancer cells and control their morphology and gene expression. Specifically, Ac(5)ManNTGc, a thiol-bearing analog of N-acetyl-d-mannosamine (ManNAc) was used to introduce thiolated sialic acids into the glycocalyx of human Jurkat T-lymphoma derived cells. In parallel 2D films and 3D electrospun nanofibrous scaffolds were prepared from polyethersulfone (PES) and (as controls) left unmodified or aminated. Alternately, the materials were malemided or gold-coated to provide bio-orthogonal binding partners for the thiol groups newly expressed on the cell surface. Cell attachment was modulated by both the topography of the substrate surface and by the chemical compatibility of the binding interface between the cell and the substrate; a substantial increase in binding for normally non-adhesive Jurkat line for 3D scaffold compared to 2D surfaces with an added degree of adhesion resulting from chemoselective binding to malemidede-derivatived or gold-coated surfaces. In addi...Continue Reading

References

Apr 29, 1994·Science·R SinghviD E Ingber
May 30, 1997·Science·C S ChenD E Ingber
Jan 9, 1999·Trends in Biotechnology·G A Lemieux, C R Bertozzi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 14, 2003·The Journal of Biological Chemistry·Shigetoshi EdaMasatoshi Beppu
Apr 2, 2004·Nature·Robert Langer, David A Tirrell
Sep 25, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yanzhong ZhangZheng-Ming Huang
Dec 24, 2005·Science Progress·George M WhitesidesJ Christopher Love
Feb 14, 2006·Nature Chemical Biology·Srinivasa-Gopalan SampathkumarKevin J Yarema
Dec 23, 2006·Chemistry & Biology·Srinivasa-Gopalan SampathkumarKevin J Yarema
Feb 20, 2007·Molecular BioSystems·Christopher T CampbellKevin J Yarema
Apr 5, 2007·Nature Protocols·Srinivasa-Gopalan SampathkumarKevin J Yarema
Sep 4, 2007·Nature Methods·Claudia FischbachDavid J Mooney
Apr 21, 2009·International Journal of Biological Macromolecules·Young-Jin KimMyung-Seok Choi
Jul 25, 2009·Journal of Cellular and Molecular Medicine·Dietmar W HutmacherUlrich Kneser
Aug 14, 2009·Glycobiology·Jian DuKevin J Yarema
Feb 2, 2010·Advanced Drug Delivery Reviews·Jian Du, Kevin J Yarema

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Citations

Jan 6, 2016·Biomaterials·Girdhari Rijal, Weimin Li
Nov 18, 2015·Chembiochem : a European Journal of Chemical Biology·Bo ChengXing Chen
Jun 1, 2013·Materials Today·Xianfeng WangBingyun Li
Jan 10, 2017·ACS Nano·James P K ArmstrongMolly M Stevens
May 28, 2016·Experimental Biology and Medicine·James Pk Armstrong, Adam W Perriman
Feb 22, 2017·Chembiochem : a European Journal of Chemical Biology·Mohit P MathewKevin J Yarema
Aug 18, 2020·Macromolecular Rapid Communications·Maria C Arno
Aug 25, 2020·Biomaterials Science·Marta CavoLoretta L Del Mercato
Aug 9, 2021·Drug Delivery and Translational Research·Gayatri Gopal ShetgaonkarRupesh Kalidas Shirodkar

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