Designing a magnetic inductive micro-electrode for virus monitoring: modelling and feasibility for hepatitis B virus.

Mikrochimica Acta
Elias AlipourPetr I Nikitin

Abstract

A simple model is designed for an inductive immunosensor in which the magnetic particles are attached to the bioreceptors to form a sandwich on the surface of an inductor. The inductor consists of a coil covered on a silicon oxide wafer. The coil comprises 250 turns of a planar gold wire, which is approximately 200 nm thick and 392 mm long, placed in a circle with a diameter of 2 mm. The model is well characterised by controlling the geometrical and electrical parameters and also the permeability of the magnetic material. To evaluate the feasibility of the model for virus monitoring, a novel inductive immunosensor is designed and for the first time applied for the detection of hepatitis B surface antigen (HBsAg). At first, Fab' segment of primary anti-HBsAg is immobilised on the coil. Then, the coil is exposed to HBsAg and the complex is introduced to a secondary antibody conjugated with magnetic particles to form an immune-sandwich. Finally, the influence of magnetic particles on the coil inductance is recorded and used as a signal for HBsAg detection. The magnetic inductive immunosensor showed specific responses toward HBsAg with the detection limit of 1 ng mL-1, linear range of 1 to 200 ng mL-1, and a sensitivity of 6 × 10-4...Continue Reading

References

Mar 2, 2005·Nature Reviews. Immunology·Barbara Rehermann, Michelina Nascimbeni
Feb 5, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Li-Juan DengJian Huang
Nov 22, 2008·Colloids and Surfaces. B, Biointerfaces·Willem M AlbersInger Vikholm-Lundin
Apr 27, 2010·Analytical Chemistry·Takahisa IbiiTakeshi Imamura
Dec 28, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jana ŠťávováAlena Kubátová
Jan 1, 2009·Sensors·Isaac Koh, Lee Josephson
Mar 8, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wai-Kay SetoMan-Fung Yuen
Jul 9, 2013·Analytical Biochemistry·Sara NouraniSeyed Mehdi Boutorabi
Sep 26, 2014·World Journal of Gastroenterology : WJG·Chun-Yan Yao, Wei-Ling Fu
Mar 31, 2015·Biosensors & Bioelectronics·Victor Crivianu-Gaita, Michael Thompson
May 4, 2016·Applied Microbiology and Biotechnology·Airis Maria Araújo MeloCarlúcio R Alves
Oct 11, 2017·Sensors·Yi-Ting ChenT Randall Lee

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.