Designing a multi-epitope based vaccine to combat Kaposi Sarcoma utilizing immunoinformatics approach

Scientific Reports
Varun ChauhanMini P Singh

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) responsible for causing Kaposi sarcoma (KS), an opportunistic angioproliferative neoplasm is emerging rapidly. Despite this there is no permanent cure for this disease. The present study was aimed to design a multi-epitope based vaccine targeting the major glycoproteins of KSHV which plays an important role in the virus entry. After the application of rigorous immunoinformatics analysis and several immune filters, the multi-epitope vaccine was constructed, consisting of CD4, CD8 and IFN-γ inducing epitopes. Several physiochemical characteristics, allergenicity and antigenicity of the multi-epitope vaccine were analyzed in order to ensure its safety and immunogenicity. Further, the binding affinity and stability of the vaccine with Toll like receptor -9 (TLR-9) was analyzed by molecular docking and dynamics simulation studies. In addition, an in silico cloning was performed to ensure the expression and translation efficiency of the vaccine, utilizing pET-28a (+) vector. Such T-cell-based immunotherapies which leverage this mechanism could prove their potential against cancer. Further, the authors propose to test the present findings in the lab settings to ensure the safety, immunoge...Continue Reading

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Methods Mentioned

BETA
glycosylation
X-ray

Software Mentioned

BLASTP
Raptor
MEGA
NetCTL
SnapGene
Immune Epitope Database ( IEDB ) Conservancy Analysis tool
web
PIPER
PYMOL
Net MHC II pan

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