Designing a multi-epitope vaccine against SARS-CoV-2: an immunoinformatics approach.

Journal of Biomolecular Structure & Dynamics
Abdus SamadMd Shahedur Rahman

Abstract

Ongoing COVID-19 outbreak has raised a drastic challenge to global public health security. Most of the patients with COVID-19 suffer from mild flu-like illnesses such as cold and fever; however, few percentages of the patients progress from severe illness to death, mostly in an immunocompromised individual. The causative agent of COVID-19 is an RNA virus known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Despite these debilitating conditions, no medication to stop the disease progression or vaccination is available till now. Therefore, we aimed to formulate a multi-epitope vaccine against SARS-CoV-2 by utilizing an immunoinformatics approach. For this purpose, we used the SARS-CoV-2 spike glycoprotein to determine the immunodominant T- and B-cell epitopes. After rigorous assessment, we designed a vaccine construct using four potential epitopes from each of the three epitope classes such as cytotoxic T-lymphocytes, helper T-lymphocyte, and linear B-lymphocyte epitopes. The designed vaccine was antigenic, immunogenic, and non-allergenic with suitable physicochemical properties and has higher solubility. More importantly, the predicted vaccine structure was similar to the native protein. Further investigations ...Continue Reading

References

Dec 1, 1995·Computer Applications in the Biosciences : CABIOS·C Geourjon, G Deléage
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·J L SussmanE E Abola
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Jan 31, 2003·Proteins·Simon C LovellDavid C Richardson
Jul 1, 1963·Journal of Molecular Biology·G N RAMACHANDRANV SASISEKHARAN
Jun 28, 2005·Nucleic Acids Research·Andreas GroteDieter Jahn
Mar 21, 2006·BMC Bioinformatics·Huynh-Hoa BuiAlessandro Sette
Jan 9, 2007·BMC Bioinformatics·Irini A Doytchinova, Darren R Flower
May 23, 2007·Nucleic Acids Research·Markus Wiederstein, Manfred J Sippl
Nov 2, 2007·BMC Bioinformatics·Mette V LarsenMorten Nielsen
Jun 25, 2009·Bioinformatics·Christophe N MagnanPierre Baldi
Oct 12, 2010·Bioinformatics·Christophe N MagnanPierre Baldi
Nov 26, 2010·BMC Bioinformatics·Peng WangBjoern Peters
Oct 19, 2011·Nucleic Acids Research·Brett E PickettRichard H Scheuermann
Sep 22, 2012·Computational and Mathematical Methods in Medicine·F CastiglioneA Prisco
Jun 14, 2013·BMC Bioinformatics·Ivan DimitrovIrini Doytchinova
Jun 12, 2013·Nucleic Acids Research·Daniel W A BuchanDavid T Jones
Aug 1, 2013·Proteins·Timothy NugentDavid T Jones
Sep 24, 2013·PloS One·Sudheer GuptaGajendra P S Raghava
Nov 10, 2013·PLoS Computational Biology·Jorg J A CalisBjoern Peters
Dec 7, 2013·Biology Direct·Sandeep Kumar DhandaGajendra P S Raghava
Feb 4, 2014·Clinical & Developmental Immunology·Sandeep Kumar DhandaG P S Raghava
Apr 29, 2014·Nucleic Acids Research·José Ramón López-BlancoPablo Chacón
Apr 30, 2015·Immune Network·Sujin Lee, Minh Trang Nguyen
Jul 28, 2015·The Journal of Clinical Investigation·Cornelis J M MeliefSjoerd H van der Burg
Jan 1, 2014·Vaccines·Weidang LiAshlesh K Murthy
Apr 24, 2016·Nucleic Acids Research·Natasha S Latysheva, M Madan Babu
Jan 13, 2017·Nature Protocols·Dima KozakovSandor Vajda
Feb 18, 2017·Scientific Reports·Gandharva NagpalGajendra P S Raghava
Jul 3, 2017·Trends in Pharmacological Sciences·Srinivasa Reddy BonamSylviane Muller
Aug 14, 2018·Frontiers in Immunology·Balachandran ManavalanGwang Lee
Dec 21, 2018·PLoS Pathogens·Judith OlejnikElke Mühlberger
Jan 13, 2019·Journal of Biomolecular Structure & Dynamics·Hesam DorostiYounes Ghasemi
Jun 18, 2019·Journal of Biomolecular Structure & Dynamics·Poghos O VardevanyanMariam A Shahinyan
Dec 25, 2019·Journal of Biomolecular Structure & Dynamics·Sunil Kumar Tripathi, Dinakar M Salunke
Jan 25, 2020·The New England Journal of Medicine·Na ZhuUNKNOWN China Novel Coronavirus Investigating and Research Team
Jan 28, 2020·Lancet·Chen WangGeorge F Gao

❮ Previous
Next ❯

Citations

Aug 11, 2020·Journal of Biomolecular Structure & Dynamics·Arpita Devi, Nyshadham S N Chaitanya
Oct 13, 2020·Nucleic Acids Research·Shuyi FangJun Wan
Nov 10, 2020·Journal of Biomolecular Structure & Dynamics· JyotishaInsaf Ahmed Qureshi
Nov 24, 2020·Journal of Biomolecular Structure & Dynamics·Zahra YazdaniReza Valadan
Dec 1, 2020·Journal of Biomolecular Structure & Dynamics·Vijaya Sai AyyagariKrupanidhi Srirama
Jan 20, 2021·Advanced Drug Delivery Reviews·Muhammad Saqib SohailMatthew R McKay
Jan 20, 2021·Journal of Biomolecular Structure & Dynamics·Uma ShankarAmit Kumar
Feb 10, 2021·Advanced Drug Delivery Reviews·Woochang HwangNamshik Han
Feb 12, 2021·Frontiers in Molecular Biosciences·Tofael Ahmed SumonEun-Woo Lee
Apr 4, 2021·Medicina·Michael DoulberisJannis Kountouras
Mar 26, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neha SangewarWaithaka Mwangi
May 27, 2021·Journal of Biomolecular Structure & Dynamics·Vikrant Singh RajputAbhinav Grover
Jul 20, 2021·International Journal of Peptide Research and Therapeutics·Chiranjib ChakrabortySang-Soo Lee
Aug 24, 2021·Biologia·Bahareh VakiliManica Negahdaripour

❮ Previous
Next ❯

Datasets Mentioned

BETA
QIC53213

Software Mentioned

BIOVIA Discovery Studio
AllerTop
AutoDock Vina script
MolProbity
YASARA
TASSER ( Refinement
VaxiJen
ProtParam
SOPMA
IMMSIM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved