Designing a sensitive and quantifiable nanocolloid assay with dielectrophoretic crossover frequencies.

Biomicrofluidics
Sagnik Basuray, Hsueh-Chia Chang

Abstract

Dielectrophoretic nanocolloid assay is a promising technique for sensitive molecular detection and identification, as target molecule hybridization onto the probe-functionalized nanocolloids can change their surface conductance and consequently their dielectrophoretic crossover frequencies. Thus, instead of relying on surface charge density increase after hybridization, as in many capacitive and field effect transistor impedance sensing techniques, the current assay utilizes the much larger surface conductance (and dielectrophoresis crossover frequency) changes to effect sensitive detection. Herein, we present a Poisson-Boltzmann theory for surfaces with finite-size molecular probes that include the surface probe conformation, their contribution to surface charge with a proper delineation of the slip and Stern planes. The theory shows that the most sensitive nanocolloid molecular sensor corresponds to a minimum in the dielectrophoretic crossover frequency with respect to the bulk concentration of the molecular probes (oligonucleotides in our case) during nanocolloid functionalization. This minimum yields the lowest number of functionalized probes that are also fully stretched because of surface probe-probe interaction. Our theo...Continue Reading

References

Mar 15, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·J P HuangL Dong
Mar 10, 2004·Lab on a Chip·Elisabeth Verpoorte
Mar 31, 2005·Journal of Colloid and Interface Science·Irina Ermolina, Hywel Morgan
Apr 20, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·T B JonesD J Yao
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Sagnik Basuray, Hsueh-Chia Chang
Aug 21, 2009·Biomicrofluidics·Satyajyoti SenapatiHsueh-Chia Chang
Oct 1, 2008·Expert Opinion on Drug Discovery·Yuan Wen, Shang-Tian Yang

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Citations

Aug 3, 2013·Biomicrofluidics·Asuka NakanoAlexandra Ros
Aug 11, 2010·Biomicrofluidics·Sagnik BasurayHsueh-Chia Chang
Aug 11, 2010·Biomicrofluidics·Miguel SanchoRonald Pethig
Aug 6, 2010·Biomicrofluidics·S AbdallaS H Al-Magaishi
Jan 10, 2014·Biomicrofluidics·Jianming SangZhihong Li
Jul 21, 2010·Biomicrofluidics·Naga Siva Kumar Gunda, Sushanta Kumar Mitra
May 16, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Rajagopalan ThiruvengadathanShubhra Gangopadhyay
Apr 10, 2010·Lab on a Chip·I-Fang ChengHsueh-Chia Chang
Jun 25, 2014·Membranes·Hirohisa Tamagawa, Sachi Morita
Feb 13, 2013·Electrophoresis·Asuka Nakano, Alexandra Ros
Jun 30, 2011·IEEE Transactions on Nanobioscience·S Abdalla
May 16, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Michal Přibyl, Dalimil Šnita
Sep 28, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Gilad Yossifon, Hsueh-Chia Chang
Feb 24, 2015·Bioanalysis·Fernanda Camacho-Alanis, Alexandra Ros

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Methods Mentioned

BETA
light scattering
DNA assay

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