Designing intracellular metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechosystis sp. 6803 genome-scale model

Microbial Cell Factories
Tomokazu ShiraiAkihiko Kondo

Abstract

Designing optimal intracellular metabolism is essential for using microorganisms to produce useful compounds. Computerized calculations for flux balance analysis utilizing a genome-scale model have been performed for such designs. Many genome-scale models have been developed for different microorganisms. However, optimal designs of intracellular metabolism aimed at producing a useful compound often utilize metabolic reactions of only the host microbial cells. In the present study, we added reactions other than the metabolic reactions with Synechosystis sp. 6803 as a host to its genome-scale model, and constructed a metabolic model of hybrid cells (SyHyMeP) using computerized analysis. Using this model provided a metabolic design that improves the theoretical yield of succinic acid, which is a useful compound. Constructing the SyHyMeP model enabled new metabolic designs for producing useful compounds. In the present study, we developed a metabolic design that allowed for improved theoretical yield in the production of succinic acid during glycogen metabolism by Synechosystis sp. 6803. The theoretical yield of succinic acid production using a genome-scale model of these cells was 1.00 mol/mol-glucose, but use of the SyHyMeP model...Continue Reading

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Citations

Jun 25, 2017·Trends in Biotechnology·Shuhei Noda, Akihiko Kondo
Mar 16, 2019·Frontiers in Bioengineering and Biotechnology·María Santos-MerinoDaniel C Ducat
Jan 4, 2017·Bioprocess and Biosystems Engineering·M A Valderrama-GomezA Kremling
May 8, 2018·Biotechnology Advances·Tao SunWeiwen Zhang
Oct 14, 2021·Critical Reviews in Biotechnology·Renan Yuji MiyamotoLeticia Maria Zanphorlin

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Software Mentioned

Synthetic
SyHyMeP
Hybrid Metabolic Pathway design tool (
OptForce
MOMA
GLPK
SyGSM
HyMeP )
Apache Tomcat
OptFlux

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