Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells

Scientific Reports
Jangho KimJong Hoon Chung

Abstract

Inspired by ultrastructural analysis of ex vivo human tissues as well as the physiological importance of structural density, we fabricated nanogrooves with 1:1, 1:3, and 1:5 spacing ratio (width:spacing, width = 550 nm). In response to the nanotopographical density, the adhesion, migration, and differentiation of human mesenchymal stem cells (hMSCs) were sensitively controlled, but the proliferation showed no significant difference. In particular, the osteo- or neurogenesis of hMSCs were enhanced at the 1:3 spacing ratio rather than 1:1 or 1:5 spacing ratio, implying an existence of potentially optimized nanotopographical density for stem cell function. Furthermore, such cellular behaviors were positively correlated with several cell morphological indexes as well as the expression of integrin β1 or N-cadherin. Our findings propose that nanotopographical density may be a key parameter for the design and manipulation of functional scaffolds for stem cell-based tissue engineering and regenerative medicine.

References

Apr 15, 2003·Journal of Cell Science·Ana I TeixeiraPaul F Nealey
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Jan 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xingyu JiangGeorge M Whitesides
Apr 9, 2005·Biomaterials·Evelyn K F YimKam W Leong
Jun 25, 2005·European Cells & Materials·G Rh OwenR G Richards
Nov 19, 2005·Science·Molly M Stevens, Julian H George
Jun 15, 2006·Tissue Engineering·Quynh P PhamAntonios G Mikos
Jul 4, 2006·Journal of Biomedical Materials Research. Part a·S J LiliensiekC J Murphy
May 15, 2007·Nature·Manuel ThéryFrank Jülicher
Sep 25, 2007·Nature Materials·Matthew J DalbyRichard O C Oreffo
May 21, 2008·Archives of Dermatology·Gary J FisherJohn J Voorhees
Aug 7, 2008·Cell Stem Cell·Lino FerreiraRobert Langer
Dec 9, 2008·Tissue Engineering. Part a·George Kirmizidis, Mark A Birch
Jan 1, 2008·Advanced Materials·Christopher J BettingerRobert Langer
May 22, 2009·Nature Materials·Elsie S PlaceMolly M Stevens
Jun 6, 2009·Angewandte Chemie·Christopher J BettingerJeffrey T Borenstein
Jul 3, 2009·Cell Stem Cell·Farshid GuilakChristopher S Chen
Sep 26, 2009·Clinical and Translational Science·Timothy J NelsonAndre Terzic
Nov 27, 2009·Nature·Matthias P LutolfHelen M Blau
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Deok-Ho KimAndre Levchenko
Jan 2, 2010·Accounts of Chemical Research·Omar Z FisherNicholas A Peppas
Feb 9, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Manus Jonathan Paul BiggsMatthew J Dalby
Feb 25, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Feng ZhaoKam W Leong
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristopher A KilianMilan Mrksich
Aug 31, 2010·Advanced Materials·Deok-Ho KimAndre Levchenko
Oct 1, 2010·Advanced Materials·Matthias P Lutolf, Helen M Blau
Feb 8, 2011·Soft Matter·Kevin J ChalutKam W Leong
Feb 26, 2011·Journal of Tissue Engineering·Laura E McNamaraMatthew J Dalby
Mar 3, 2011·Cell Stem Cell·Gordana Vunjak-Novakovic, David T Scadden
Nov 15, 2011·Nature Cell Biology·Masha Prager-KhoutorskyAlexander D Bershadsky
Feb 16, 2012·Nature Communications·Marie VersaevelSylvain Gabriele
Mar 14, 2012·Advanced Materials·Pinar ZorlutunaAli Khademhosseini
Apr 21, 2012·Biomaterials·Karina KulangaraKam W Leong
May 2, 2012·The Journal of Cell Biology·Deok-Ho KimAndre Levchenko
May 25, 2012·Tissue Engineering. Part C, Methods·Jangho KimJong Hoon Chung
Jul 20, 2012·Annual Review of Biomedical Engineering·Mónica Beato CoelhoJeffrey M Karp
Aug 28, 2012·Advanced Drug Delivery Reviews·Hong Nam KimKahp-Yang Suh
Sep 11, 2012·Biomaterials·Hong Nam KimKahp-Yang Suh
Nov 10, 2012·ACS Nano·Chaenyung ChaNicholas A Peppas

❮ Previous
Next ❯

Citations

Dec 3, 2014·Advanced Drug Delivery Reviews·J LeijtenF P Luyten
Feb 18, 2016·Stem Cells International·Azizeh-Mitra YousefiHassane Oudadesse
Oct 3, 2015·ACS Applied Materials & Interfaces·Roey ElnathanNicolas H Voelcker
Jan 14, 2016·IEEE Transactions on Nanobioscience·Lee-Woon JangJungkyu Kim
Nov 8, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jangho KimJong Hoon Chung
Aug 8, 2015·BioMed Research International·Shimaa A AbdellatefAkiyoshi Taniguchi
Jul 9, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Alexey KlymovX Frank Walboomers
Dec 10, 2015·Journal of Biomedical Materials Research. Part a·Robeth Viktoria ManurungSurojit Chattopadhyay
Jan 18, 2017·Journal of Biomedical Materials Research. Part a·Kanika VatsDanielle S W Benoit
Jul 28, 2016·Scientific Reports·Ki-Hwan NamDeok-Ho Kim
Oct 1, 2016·Journal of Tissue Engineering and Regenerative Medicine·Sharanya SankarSeeram Ramakrishna
Dec 28, 2017·Advanced Healthcare Materials·Jiao Jiao LiHala Zreiqat
Jan 13, 2018·Journal of Tissue Engineering and Regenerative Medicine·Sharanya SankarSubha N Rath
Jul 5, 2018·Journal of Investigative and Clinical Dentistry·Gaia PellegriniMassimo Del Fabbro
Sep 26, 2017·Connective Tissue Research·Gyudo LeeJeffrey J Fredberg
Mar 13, 2014·Tissue Engineering. Part a·Jangho KimAbhay Pandit
Mar 26, 2019·Advanced Materials·Benjamin R Freedman, David J Mooney
Nov 12, 2015·Bone Research·Tao GongYunfeng Lin
Sep 29, 2019·Clinical Hemorheology and Microcirculation·Jie ZouAndreas Lendlein
Jul 4, 2018·Proceedings of the National Academy of Sciences of the United States of America·Hon Fai ChanXuanhe Zhao
Jun 22, 2017·Advanced Healthcare Materials·Jangho KimJong Hoon Chung
Nov 9, 2019·Microsystems & Nanoengineering·Junghoon KimSungWoo Nam
Nov 6, 2020·Biomaterials Science·Kamol DeyLuciana Sartore
Jan 27, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Jeffrey A HorowitzStephen R Forrest
Dec 29, 2020·Current Opinion in Solid State & Materials Science·Wei ZhangBianxiao Cui
Oct 30, 2020·Frontiers in Bioengineering and Biotechnology·Raymond TranCorinne A Hoesli
Feb 28, 2021·Journal of Biomedical Materials Research. Part a·A Hernández-Rangel, E San Martin-Martinez

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA

Software Mentioned

MATLAB

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.