Designing of enzyme inhibitors based on active site specificity: lessons from methyl gallate and its lipoxygenase inhibitory profile

Journal of Receptor and Signal Transduction Research
Sharanya C SHaridas M

Abstract

Methyl gallate was purified, by lipoxygenase (LOX) inhibitory activity-guided method since its alleged anti-inflammatory property, from Bergenia ligulata (Wall), a plant used in the traditional, Ayurvedic system of medicine extensively. The LOX inhibitory property of methyl gallate was studied by enzyme kinetics, isothermal titration calorimetry and molecular docking followed by molecular simulation studies. The wet-laboratory experiments and in silico studies showed complete agreement, and promise of methyl gallate as a drug-lead molecular scaffold for anti-inflammatory therapy, based on LOX inhibition. The expressed work shows the need of nonactive site binding parameters to be considered while designing of inhibitors based on the specificities toward active sites of enzymes.

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Citations

Sep 14, 2019·Journal of Receptor and Signal Transduction Research·Goverdhan LankaSarita Rajender Potlapally
Oct 11, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Luana Barbosa CorreaElaine Cruz Rosas

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