Designing of Nucleocapsid Protein Based Novel Multi-epitope Vaccine Against SARS-COV-2 Using Immunoinformatics Approach

International Journal of Peptide Research and Therapeutics
Janish KumarInsaf Ahmed Qureshi

Abstract

The COVID-19 disease is caused by SARS-CoV-2 and spreading rapidly worldwide with extremely high infection rate. Since effective and specific vaccine is not available to combat the deadly COVID-19, the objective of our study was to design a multi-epitope vaccine using immunoinformatics approach with translational implications. Nucleocapsid (N) protein of SARS-CoV-2 is stable, conserved and highly immunogenic along with being less prone to mutations during infection, which makes it a suitable candidate for designing vaccine. In our study, B- and T-cells epitopes were identified from N protein and screened based on crucial parameters to design the multi-epitope vaccine construct. Additionally, human beta-defensin-2 was incorporated into the vaccine construct as an adjuvant along with suitable linkers followed by its further evaluation based on crucial parameters including allergenicity, antigenicity, stability etc. Combined major histocompatibility complexes (MHC-I and MHC-II) binding epitopes presented broader population coverage of the vaccine throughout the world. The three-dimensional structure of vaccine candidate implied strong interaction with toll-like receptor 3 (TLR3) using molecular docking. The vaccine-TLR3 complex wa...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·J L PellequerM H Van Regenmortel
Jun 25, 2004·The Journal of Infectious Diseases·Danny Tze Ming LeungPak Leong Lim
Jun 17, 2008·International Journal of Pharmaceutics·Yvonne PerrieVincent W Bramwell
Oct 3, 2012·Advanced Drug Delivery Reviews·Xiaoying ChenWei-Chiang Shen
May 23, 2014·Immunologic Research·Rudragouda ChannappanavarStanley Perlman
May 23, 2014·Journal of Chemical Information and Modeling·Rashmi KumariAndrew Lynn
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Jun 15, 2018·Journal of Cellular Biochemistry·Rajan Kumar PandeyVijay Kumar Prajapati
Feb 3, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Tung Phan
Mar 29, 2020·Current Tropical Medicine Reports·Wen-Hsiang ChenMaria Elena Bottazzi
May 15, 2020·F1000Research·Chloe H Lee, Hashem Koohy
Apr 17, 2020·Journal of Biomolecular Structure & Dynamics·Maryam EnayatkhaniKhadijeh Ahmadi
May 8, 2020·Microbial Pathogenesis·Parismita KalitaTimir Tripathi
May 27, 2020·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sajjad AhmadSyed Sikander Azam
Jun 18, 2020·Journal of Virology·Noton K DuttaJames T Gordy
Jul 15, 2020·Journal of Virology·Gustaf AhlénUNKNOWN representing the OPENCORONA consortia
Nov 10, 2020·Journal of Biomolecular Structure & Dynamics· JyotishaInsaf Ahmed Qureshi

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
protein folding

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.