Designing Peptide Bunches on Nanocage for Bispecific or Superaffinity Targeting

Biomacromolecules
Sooji KimSoyoun Kim

Abstract

Ferritin cage nanoparticles are promising platforms for targeted delivery of imaging and therapeutic agents because their cage structure can accommodate small molecules and their surfaces can be decorated with multiple functionalities. However, selective targeting is still a challenge for translating ferritin-based nanomedicines into the clinic, especially for heterogeneous diseases such as cancer. Targeting peptides can be genetically fused onto the surface of a ferritin cage, forming peptide bunches on nanocages (PBNCs) that offer synergistic increases in binding avidity. Here, we utilized two sites of the ferritin monomer, the N-terminus and the loop between the fourth and fifth helices, which are exposed on the surface of the assembled 24-subunit ferritin cage, to ligate one or two types of peptides to achieve "super affinity" and bispecificity, respectively. PBNCs formed by ligation of the IL-4 receptor-targeting peptide, AP1, to both sites (48AP1-PBNCs) tethered IL-4R, expressing tumor cells with greater affinity than did PBNCs with AP1 ligated to a single site (24AP1-PBNCs). Moreover, bispecific PBNCs containing 24 RGD peptides and 24 AP1 peptides (24RGD/24AP1-PBNCs) were capable of independently targeting cells expressi...Continue Reading

References

Jul 1, 1995·Journal of Inorganic Biochemistry·S PeadG D Watt
Apr 21, 1999·QJM : Monthly Journal of the Association of Physicians·C M EdwardsS R Bloom
Oct 28, 2003·Inorganic Chemistry·José M Domínguez-Vera, Enrique Colacio
Dec 21, 2006·Journal of the American Chemical Society·Masaki UchidaTrevor Douglas
Jan 27, 2007·Chemistry & Biology·Seungpyo HongMark M Banaszak Holl
Jul 3, 2009·Small·Aihui MahamYuehe Lin
Dec 23, 2009·Biochimica Et Biophysica Acta·Masaki UchidaTrevor Douglas
Jan 1, 2008·Drug Development Research·Zhaofei LiuXiaoyuan Chen
Nov 9, 2010·Journal of Molecular Biology·Teemu HaikarainenAnastassios C Papageorgiou
Jan 11, 2013·Journal of the Royal Society, Interface·Tais A P F DollPeter Burkhard
Nov 8, 2013·ACS Nano·Jong-Hwan LeeJeewon Lee
Nov 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Jae-A HanSebyung Kang
Dec 4, 2013·Vaccine·Liang ZhaoAnton P J Middelberg
Feb 20, 2014·Metallomics : Integrated Biometal Science·Douglas B Kell, Etheresia Pretorius
May 27, 2014·Nanoscale·Lise Schoonen, Jan C M van Hest
Oct 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Minmin LiangXiyun Yan
Dec 9, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Minsu KwonByung-Heon Lee
Jan 13, 2015·New Biotechnology·Didi He, Jon Marles-Wright
May 17, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Lianhua ChiByung-Heon Lee
Dec 10, 2015·Nanomedicine·Amar Bt Ghisaidoobe, Sang J Chung

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Citations

Dec 13, 2016·Nanoscale·Valeria de TurrisPaola Baiocco
May 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hanchun YaoBin Du
Sep 9, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jiuyang HeXiyun Yan
Mar 4, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rongguang SongWei Xue
Mar 7, 2021·International Journal of Molecular Sciences·Que Dan NguyenTakafumi Ueno

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