Designing two-in-one antibodies

Immunotherapy
Ignacio Garcia Valladares, Luis R Espinoza

Abstract

Evaluation of: Bostrom J, Shang-Fan Y, Kan D et al.: Variants of the antibody Herceptin that interact with HER2 and VEGF at the antigen binding site. Science 323, 1610-1614 (2009). The longstanding held notion that one antibody equals one antigen and, hence, one function has been challenged in recent years. Improved technology in antibody production, especially the accumulation of sequence data of immunoglobulin genes and the advent of PCR have made it possible to clone antibody gene repertoires. The current paper provides further challenge to the notion of one antibody = one antigen by developing 'two-in-one' antibodies with an antigen-binding site that binds two distinct proteins with high affinity. A therapeutic variant antibody of Herceptin (Genentech, CA, USA) was isolated that binds the human EGF receptor (HER)2 and also to VEGF. This development may represent a breakthrough discovery and may have significant implications in the therapy of malignant, infectious, allergic and autoimmune disorders.

References

Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·J Foote, C Milstein
Mar 1, 2003·Science·Jefferson Foote
Mar 1, 2003·Science·Leo C JamesDan S Tawfik
Apr 19, 2006·Immunity·Roy A Mariuzza
Apr 24, 1942·Science·L Pauling, D H Campbell
Mar 3, 2009·Methods in Molecular Biology·Limor Nahary, Itai Benhar
Mar 21, 2009·Science·Paul W H I Parren, Dennis R Burton

❮ Previous
Next ❯

Citations

Jan 31, 2021·Nature Communications·Roland BeckmannHubert Kettenberger

❮ Previous
Next ❯

Methods Mentioned

BETA
xenograft
PCR

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.