PMID: 9429157Jan 16, 1998Paper

Desmin-lacZ transgene expression and regeneration within skeletal muscle transplants

Journal of Muscle Research and Cell Motility
L LescaudronJ Fontaine-Pérus

Abstract

The purpose of this study was to investigate the initiation and time course of the regeneration process in fragments of skeletal muscle transplants as a function of muscle tissue age at implantation. The appearance of desmin occurs at the very beginning of myogenesis. The transgenic desmin nls lacZ mice used in the study bear a transgene in which the 1 kb DNA 5' regulatory sequence of the desmin gene is linked to a reporter gene coding for Escherichia coli beta-galactosidase. The desmin lacZ transgene labels muscle cells in which the desmin synthesis programme has commenced. We implanted pectoralis muscle fragments from fetal transgenic embryos and mature and old transgenic mice into mature non-transgenic mice. Early events of myogenesis occurring during regeneration started sooner in transplants from 4-month-old (day 3 post-implantation) muscle than in those from 24-month-old (day 5-6 post-implantation) muscle, and they lasted longer in those from young (day 17 post-implantation) than in those from old (day 14 post-implantation) muscle fragments. In adult muscle, transgene activation proceeded from the periphery toward the centre of the transplant. In transplants from fetal 18-day-old pectoralis, myotubes with transgene activi...Continue Reading

Citations

Mar 13, 2013·Scientific Reports·Silvia CarosioAntonio Musarò
Jun 28, 2014·BioMed Research International·Alessandra CostaBianca Maria Scicchitano
Oct 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J D WhiteM D Grounds
Aug 20, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jessica L StaibScott K Powers
Aug 6, 2011·Molecular Endocrinology·Angelica ToschiBianca Maria Scicchitano
Nov 3, 2006·The Journal of Clinical Investigation·Foteini MourkiotiNadia Rosenthal
Feb 1, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura PelosiAntonio Musarò
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Antonio MusaròNadia Rosenthal
Dec 11, 1999·Current Opinion in Neurology·M D Grounds
Aug 5, 2005·Journal of Cell Science·Seumas McCroskeryRavi Kambadur
Jun 11, 1999·Experimental Gerontology·A Musarò, N Rosenthal

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