PMID: 2491877Jan 1, 1989Paper

Desmoglein II-derived glycopeptides in human epidermis

The Journal of Investigative Dermatology
I A KingP R Fryer

Abstract

Antisera raised against a major 78 kD glycopeptide from pig epidermis were used to identify desmoglein II-derived glycopeptides in the conA-binding material isolated from human epidermis. In whole CaCl2-separated epidermis the antiserum recognized conA-binding components with apparent Mr of 115, 100, 82, 68, 50, 48, and 46 kD. The 82, 68, 48, and 46 kD immunoreactive bands were present in normal stratum corneum and plantar callus. Psoriatic scales contained significantly more of the 82 kD components and less of the 48 and 46 kD bands. Psoriatic scales also contained a major 50 kD conA-binding component unrelated to keratins or desmoglein II. Proteolytic peptide mapping showed that the major immunoreactive bands in normal stratum corneum and plantar callus were also chemically related. The 82 to 46 kD immunoreactive glycopeptides in plantar callus coincided with the major coomassie blue stained bands and were homogeneous on two-dimensional gels suggesting that this tissue may be a valuable source of human desmoglein II-derived glycopeptides. An antiserum directed against the electrophoretically co-purified 48/46 kD glycopeptides from plantar callus recognized the 82 to 46 kD bands in immunoblotting. In indirect immunofluorescenc...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1974·The Journal of Cell Biology·C J Skerrow, A G Matoltsy
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jul 1, 1981·The Journal of Cell Biology·G Gorbsky, M S Steinberg
Jan 1, 1984·The Journal of Investigative Dermatology·M M BryskA A Hebert
Jan 1, 1984·Journal of Cellular Biochemistry·G J GiudiceM S Steinberg

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Citations

Aug 15, 1997·Microscopy Research and Technique·M Fartasch
Jun 1, 1994·The British Journal of Dermatology·H E KnaggsW J Cunliffe
Jan 1, 1990·Archives of Dermatological Research·S J Chapman, A Walsh
Oct 13, 2011·Cancer Research·Ines BeyerAndré Lieber
Apr 1, 1993·The Journal of Investigative Dermatology·I A KingA I Magee
Jun 1, 1992·Seminars in Cell Biology·R S Buxton, A I Magee

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