Desmoglein isoform distribution affects stratum corneum structure and function

The Journal of Cell Biology
P M EliasJ R Stanley

Abstract

Desmogleins are desmosomal cadherins that mediate cell-cell adhesion. In stratified squamous epithelia there are two major isoforms of desmoglein, 1 and 3, with different distributions in epidermis and mucous membrane. Since either desmoglein isoform alone can mediate adhesion, the reason for their differential distribution is not known. To address this issue, we engineered transgenic mice with desmoglein 3 under the control of the involucrin promoter. These mice expressed desmoglein 3 with the same distribution in epidermis as found in normal oral mucous membranes, while expression of other major differentiation molecules was unchanged. Although the nucleated epidermis appeared normal, the epidermal stratum corneum was abnormal with gross scaling, and a lamellar histology resembling that of normal mucous membrane. The mice died shortly after birth with severe dehydration, suggesting excessive transepidermal water loss, which was confirmed by in vitro and in vivo measurement. Ultrastructure of the stratum corneum showed premature loss of cohesion of corneocytes. This dysadhesion of corneocytes and its contribution to increased transepidermal water loss was confirmed by tape stripping. These data demonstrate that differential ex...Continue Reading

References

Sep 1, 1989·Journal of Dental Research·C A LeschP Speight
Dec 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·H IshikawaJ Uitto
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J M CarrollL B Taichman
Jan 10, 1998·The Journal of Investigative Dermatology·Y ShirakataK Hashimoto
Jan 1, 1997·Skin Pharmacology : the Official Journal of the Skin Pharmacology Society·G K Menon, P M Elias
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·C TselepisD Garrod
Feb 18, 1999·The Journal of Clinical Investigation·M G MahoneyJ R Stanley
Jan 5, 2000·The Journal of Investigative Dermatology·I E EkholmT Egelrud
Jul 7, 2000·The New England Journal of Medicine·H WuJ R Stanley

❮ Previous
Next ❯

Citations

Apr 4, 2008·Histochemistry and Cell Biology·Jens Waschke
Dec 4, 2003·Current Opinion in Cell Biology·Julie Segre
Sep 17, 2002·Current Opinion in Cell Biology·David R GarrodZhuxiang Nie
Aug 24, 2011·Nature Reviews. Molecular Cell Biology·Cory L SimpsonKathleen J Green
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Emmanuella DelvaAndrew P Kowalczyk
Dec 16, 2003·Molecular and Cellular Biology·Xing ChengPeter J Koch
Jul 31, 2010·Dermatology Research and Practice·Gethin R Owen, David L Stokes
Mar 26, 2013·The Journal of Clinical Investigation·Christoph M Hammers, John R Stanley
Dec 25, 2012·Therapeutic Delivery·Bharti SapraAshok K Tiwary
Aug 21, 2013·International Journal of Trichology·Pramod Kumar Nigam
Jul 31, 2010·Dermatology Research and Practice·David Garrod
May 24, 2012·Journal of Allergy·Mary Beth HoganNevin W Wilson
Nov 25, 2004·Seminars in Cell & Developmental Biology·Taofei Yin, Kathleen J Green
Apr 23, 2003·The Journal of Cell Biology·Stefan BonnéFrans van Roy
Jun 24, 2009·The Journal of Cell Biology·Spiro GetsiosKathleen J Green
Jan 28, 2014·Cell Communication & Adhesion·Mohamed Berika, David Garrod
May 9, 2013·The Journal of Investigative Dermatology·Etienne TokonzabaPeter J Koch
Oct 20, 2009·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Chu SatoToshiro Tagawa
Aug 8, 2002·The Journal of Investigative Dermatology·Yasushi HanakawaJohn R Stanley
Mar 22, 2003·The Journal of Investigative Dermatology·Ken Ishii
Apr 9, 2010·The Journal of Investigative Dermatology·Thomas BiedermannErnst Reichmann
Dec 18, 2002·The Journal of Investigative Dermatology·Yasushi HanakawaKoji Hashimoto
Feb 13, 2008·The Journal of Investigative Dermatology·Eliane J MüllerMaja M Suter
Apr 8, 2006·The Journal of Investigative Dermatology·Jean-Pierre HachemPeter M Elias
Jul 26, 2003·The Journal of Investigative Dermatology·Jean-Pierre HachemPeter M Elias
Jul 13, 2004·The Journal of Investigative Dermatology·Jean JaubertJulia A Segre
Oct 16, 2007·The Journal of Investigative Dermatology·Kathleen J Green, Cory L Simpson
Sep 4, 2007·Journal of Dermatological Science·Yasushi KikuchiYasufumi Kaneda
Oct 11, 2015·Journal of Clinical Immunology·Gábor MócsaiAndrea Szegedi
Mar 9, 2005·Oral Diseases·C ScullyS R Porter
Jun 10, 2004·The Journal of Dermatology·Xing Cheng, Peter J Koch
Jan 31, 2006·Journal of the European Academy of Dermatology and Venereology : JEADV·M DavidY Milner
Dec 2, 2008·Experimental Dermatology·Ehrhardt ProkschJens-Michael Jensen
Oct 13, 2011·The Journal of Pathology·Matthew A BrookeDavid P Kelsell
Aug 15, 2009·The British Journal of Dermatology·F CaponK E Harman
Apr 12, 2005·European Journal of Cell Biology·Xing ChengPeter J Koch
Jul 30, 2015·Journal of Immunological Methods·Lucille AdamFrédéric Martinon
Sep 15, 2007·Biochimica Et Biophysica Acta·David Garrod, Martyn Chidgey
Dec 21, 2004·Differentiation; Research in Biological Diversity·Hendrik UyttendaeleAngela M Christiano
Dec 21, 2004·Differentiation; Research in Biological Diversity·Donna BrennanMy G Mahoney
Mar 13, 2003·Experimental Dermatology·L PulkkinenM G Mahoney

❮ Previous
Next ❯

Datasets Mentioned

BETA
U86016

Methods Mentioned

BETA
transfection
transgenic
Genotyping
PCR
transgenics
biopsies

Software Mentioned

ImageQuant

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.