Desorption electrospray ionization mass spectrometry analysis of lipids after two-dimensional high-performance thin-layer chromatography partial separation.

Analytical Chemistry
Giuseppe PagliaR Graham Cooks

Abstract

Molecular imaging of separate but still incompletely resolved spots on high-performance thin-layer chromatography (HPTLC) plates is used for the direct analysis of porcine brain lipids by desorption electrospray ionization mass spectrometry (DESI-MS). Eight class-specific spots were imaged in the negative ion mode and shown to contain more than fifty lipids. A low lateral resolution of 400 x 400 microm allowed simple, rapid, and incomplete separation to be combined with DESI imaging for the identification of many components of these extremely complex mixtures. In this work, tandem mass spectrometry (MS/MS) was also employed to confirm the identity of particular lipids directly on HPTLC plates.

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Citations

Nov 6, 2010·Analytical and Bioanalytical Chemistry·Min LiHuwei Liu
Apr 6, 2011·Analytical and Bioanalytical Chemistry·Andrea PennaGiovanni Predieri
Jul 7, 2011·Analytical and Bioanalytical Chemistry·Luciane C AlbericiRosana M Alberici
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Apr 22, 2015·Analytical and Bioanalytical Chemistry·Giuseppe PagliaGiuseppe Astarita
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Nov 13, 2013·Analytical Chemistry·Min LiHuwei Liu
Mar 20, 2014·Analytical Chemistry·Giuseppe PagliaGiuseppe Astarita

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