PMID: 11342164May 9, 2001Paper

Destabilization of neutrophil NADPH oxidase by ATP and other trinucleotides and its prevention by Mg(2+)

Biochimica Et Biophysica Acta
M TamuraT Kai

Abstract

Neutrophil NADPH oxidase (O(2)(-) generating enzyme) activated in a cell-free system was deactivated by dilution. When ATP was included in dilution the deactivation was further accelerated. The deactivation by dilution was biphasic, and the half-life of the enzyme was significantly shortened by ATP in each phase. ADP and AMP had little effect on the enzyme longevity while GTP and CTP had a similar effect to ATP. Staurosporine, a wide-range inhibitor of protein kinases, had no effect on ATP-induced deactivation, suggesting that the effect was not due to a protein phosphorylation. Mg(2+) addition largely prevented the deactivation by ATP. Chemical crosslinking of the activated oxidase prevented the deactivation by dilution and ATP, suggesting that the deactivation is caused by dissociation of the oxidase complex. Estimation of actin filament (F-actin) showed that the F-actin level was markedly reduced by addition of ATP. The ATP effect on the deactivation was not prominent in a semi-recombinant system which does not contain cytosol. These results suggest that ATP-induced deactivation is largely due to the chelation of Mg(2+) and are consistent with the concept that Mg(2+) stabilizes the oxidase complex by stabilizing F-actin.

References

Mar 23, 1978·The New England Journal of Medicine·B M Babior
Feb 1, 1987·Journal of Cellular Biochemistry·J Katz, P A Wals
Nov 2, 1995·Biochemical and Biophysical Research Communications·M YamaguchiS Ishibashi
Oct 21, 1994·Biochimica Et Biophysica Acta·A J ThrasherA W Segal
Dec 1, 1996·Journal of Leukocyte Biology·F R DeLeo, M T Quinn
Jan 3, 1997·Biochemical and Biophysical Research Communications·T MorimatsuM Tamura
Nov 13, 1998·The Journal of Biological Chemistry·D MeksuriyenK Igarashi

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Citations

Mar 30, 2002·Critical Reviews in Oncology/hematology·J Anastassopoulou, T Theophanides
Apr 22, 2014·Free Radical Biology & Medicine·Hana InoueMasato Konishi
Oct 7, 2004·Archives of Biochemistry and Biophysics·Kei MiyanoMinoru Tamura
Sep 27, 2020·Antioxidants·Man Liu, Samuel C Dudley
Jul 16, 2003·Archives of Biochemistry and Biophysics·Teruaki NagasawaMinoru Tamura

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