Destabilization of the RB tumor suppressor protein and stabilization of p53 contribute to HPV type 16 E7-induced apoptosis

Virology
D L JonesK Munger

Abstract

Cells that express the human papillomavirus (HPV) type 16 E7 oncoprotein are predisposed to undergo apoptosis. Transgenic mice that have E7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. We established a cell culture system to study this process and have determined the domains of E7 that are required for predisposing cells to undergo apoptosis in response to growth arrest signals. Regions within the core pRB binding site of E7 were necessary but not sufficient for inducing apoptosis. Residues within the adenovirus conserved region 1 homology domain and the consensus casein kinase II phosphorylation site are also important for this effect on cell viability. Our data also demonstrate that the ability of E7 to induce destabilization of pRB and stabilization of p53 coincides with E7-mediated transformation and apoptosis.

References

Apr 1, 1977·Science·W W NicholsS A Dwight
Apr 1, 1992·Journal of Virology·W C PhelpsP M Howley
Aug 1, 1992·The Journal of General Virology·R C Davies, K H Vousden
Sep 24, 1992·Nature·T JacksR A Weinberg
Sep 24, 1992·Nature·A R ClarkeH te Riele
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L RaoE White
Apr 3, 1992·Cell·G I EvanD C Hancock
Jul 2, 1992·Nature·D P Lane
Nov 22, 1991·Science·H zur Hausen
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J M FirzlaffR N Eisenman
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D J McCanceL A Laimins
Jun 1, 1989·Journal of Virology·C Edmonds, K H Vousden
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·A AlmasanG M Wahl
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Q QinP D Adams
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Wu, A J Levine
May 25, 1993·Biochimica Et Biophysica Acta·K Münger, W C Phelps
Feb 7, 1997·Cell·M D JacobsonM C Raff
May 1, 1997·Journal of Virology·E S HickmanK H Vousden
Jul 15, 1997·Genes & Development·A C PhillipsK H Vousden

❮ Previous
Next ❯

Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jeremy P KamilDonald M Coen
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth A WhitePeter M Howley
Jun 19, 2007·Human Gene Therapy·Katie L GreenKevin Gaston
Feb 8, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Lena ThyrellDan Grandér
May 25, 2012·Journal of Virology·Elizabeth E HoskinsSusanne I Wells
Sep 15, 2007·Journal of Virology·Marie L NguyenJohn A Blaho
May 22, 2002·Journal of Virology·Frederick A Dick, Nicholas J Dyson
Sep 13, 2003·Journal of Virology·Wanxia HeChris Fisher
Oct 14, 2004·Journal of Virology·Karl MüngerKyungwon Huh
Feb 19, 2009·Cancer Research·Trisha M Wise-DraperSusanne I Wells
Jul 12, 2007·PLoS Biology·Boerries BrandenburgJames M Hogle
Aug 23, 2012·PloS One·Águeda Buitrago-PérezRamón García-Escudero
Nov 12, 2003·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Marina Jovanović, Dordije Karadaglić
Mar 21, 2002·The Lancet Oncology·B Abdulkarim, J Bourhis
Oct 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yoshihiro NakataniAnna M Herlitz
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S SongP F Lambert
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Brett R JohnsonBrian K Kennedy
Sep 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Katerina StratiPaul F Lambert
Aug 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kyung-Won HuhKarl Münger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis