Destabilizing domains derived from the human estrogen receptor.

Journal of the American Chemical Society
Yusuke MiyazakiThomas J Wandless

Abstract

Methods to rapidly and reversibly perturb the functions of specific proteins are desirable tools for studies of complex biological processes. We have demonstrated an experimental strategy to regulate the intracellular concentration of any protein of interest by using an engineered destabilizing protein domain and a cell-permeable small molecule. Destabilizing domains have general utility to confer instability to a wide range of proteins including integral transmembrane proteins. This study reports a destabilizing domain system based on the ligand binding domain of the estrogen receptor that can be regulated by one of two synthetic ligands, CMP8 or 4-hydroxytamoxifen.

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Citations

Feb 7, 2016·Journal of Immunological Methods·M BatonickM P Weiner
Oct 27, 2015·ACS Chemical Biology·Christopher L MooreMatthew D Shoulders
Dec 19, 2012·Trends in Biotechnology·Simon Ausländer, Martin Fussenegger
Mar 27, 2015·Journal of the Royal Society, Interface·Zoltán KisRob Krams
Jun 15, 2014·International Journal for Parasitology·Yuk-Chien Liu, Upinder Singh
Jun 1, 2016·ACS Chemical Biology·Raul NavarroThomas J Wandless
Nov 8, 2016·Nature Chemical Biology·Basudeb MajiAmit Choudhary
Nov 28, 2017·Annual Review of Genetics·Toyoaki Natsume, Masato T Kanemaki
May 28, 2016·Nature Communications·Yusuke MiyazakiThomas J Wandless
Mar 6, 2019·Angewandte Chemie·Debasish MannaAmit Choudhary
Oct 26, 2013·The Journal of Biological Chemistry·Bernard W ChuThomas J Wandless
Feb 8, 2020·International Journal of Molecular Sciences·Weiye ZhaoFu-Sen Liang
Jan 9, 2015·Expert Review of Vaccines·Oliwia AndriesRon Weiss
May 20, 2020·Angewandte Chemie·Laura M LuhPhilipp M Cromm
Jan 4, 2021·Cellular and Molecular Life Sciences : CMLS·Yu-Fan ChuangGuei-Sheung Liu
Oct 30, 2020·Frontiers in Immunology·Mohammadreza Azangou-KhyavyJafar Kiani
Nov 14, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sean Mc CaffertyNiek N Sanders
Jul 2, 2020·Cell Reports·Víctor López Del AmoAmit Choudhary
Apr 9, 2021·Nature Methods·Samuel J Hendel, Matthew D Shoulders
Jun 15, 2021·Journal of the American Chemical Society·Amy RyanAlexander Deiters
Jun 21, 2021·Signal Transduction and Targeted Therapy·Chenya ZhuoMingqiang Li
Aug 5, 2017·Chemical Reviews·George M Burslem, Craig M Crews
Mar 29, 2018·ACS Synthetic Biology·Nicole M L Wong, Wilson W Wong
Dec 11, 2021·Advanced Healthcare Materials·Ciana L LópezPatrick S Stayton
Oct 3, 2020·Cell Chemical Biology·Yusaku MiyamaeThomas J Wandless

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