PMID: 7541749Mar 1, 1995Paper

Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions

Electrophoresis
G Sreeramulu, N K Singh

Abstract

A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (< 0.1 M) salt solutions were unsuitable. The method affords the advantage that expensive organic solvents, such as methanol, acetic acid or trichloroacetic acid, are not needed. Furthermore, salt destaining results in darker purple-blue protein bands as compared to the pale blue color with e.g. methanol/acetic acid destaining.

References

Oct 1, 1989·Analytical Biochemistry·B D ZehrR E Hall
Dec 1, 1985·Analytical Biochemistry·S J Compton, C G Jones
May 14, 1963·Biochimica Et Biophysica Acta·S Fazekas de St GrothA Datyner
Nov 1, 1985·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N K Singh, K W Shepherd

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Citations

Jun 21, 2011·Journal of Chemical Biology·Victoria J GauciJens R Coorssen
Apr 27, 2002·Applied and Environmental Microbiology·Oddur Vilhelmsson, Karen J Miller
Feb 1, 1997·Genome Génome / Conseil National De Recherches Canada·G Sreeramulu, N K Singh
Jan 29, 2013·Biochimica Et Biophysica Acta·Kevser BiberogluOksana Lockridge
Sep 29, 2007·Toxicon : Official Journal of the International Society on Toxinology·Yi-Rong WangJia-Qin Chen
Feb 23, 2013·Journal of Proteomics·Victoria J GauciJens R Coorssen

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